Department of Biology & Biochemistry, University of Bath, Bath, United Kingdom
Victoria E Prince
Committee on Development, Regeneration and Stem Cell Biology, The University of Chicago, Chicago, United States; Department of Organismal Biology and Anatomy, The University of Chicago, Chicago, United States
Katie Bentley
Cellular Adaptive Behaviour Lab, Francis Crick Institute, London, United Kingdom; Department of Informatics, King's College London, London, United Kingdom
Coordination of cell proliferation and migration is fundamental for life, and its dysregulation has catastrophic consequences, such as cancer. How cell cycle progression affects migration, and vice versa, remains largely unknown. We address these questions by combining in silico modelling and in vivo experimentation in the zebrafish trunk neural crest (TNC). TNC migrate collectively, forming chains with a leader cell directing the movement of trailing followers. We show that the acquisition of migratory identity is autonomously controlled by Notch signalling in TNC. High Notch activity defines leaders, while low Notch determines followers. Moreover, cell cycle progression is required for TNC migration and is regulated by Notch. Cells with low Notch activity stay longer in G1 and become followers, while leaders with high Notch activity quickly undergo G1/S transition and remain in S-phase longer. In conclusion, TNC migratory identities are defined through the interaction of Notch signalling and cell cycle progression.
