HPLC-ESI-MS method for C60 fullerene mitochondrial content quantification
Anna Grebinyk,
Sergii Grebinyk,
Svitlana Prylutska,
Uwe Ritter,
Olga Matyshevska,
Thomas Dandekar,
Marcus Frohme
Affiliations
Anna Grebinyk
Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Hochschulring 1, 15745 Wildau, Germany; Dept. of Bioinformatics, Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, Germany; Educational and Scientific Center ''Institute of Biology and Medicine'', Taras Shevchenko National University of Kyiv, Volodymyrska 64, 01601 Kyiv, Ukraine
Sergii Grebinyk
Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Hochschulring 1, 15745 Wildau, Germany
Svitlana Prylutska
Dept. of Chemistry, Taras Shevchenko National University of Kyiv, Volodymyrska 64, 01601 Kyiv, Ukraine
Uwe Ritter
Institute of Chemistry and Biotechnology, University of Technology Ilmenau, WeimarerStraße 25 (Curiebau), 98693 Ilmenau, Germany
Olga Matyshevska
Educational and Scientific Center ''Institute of Biology and Medicine'', Taras Shevchenko National University of Kyiv, Volodymyrska 64, 01601 Kyiv, Ukraine
Thomas Dandekar
Dept. of Bioinformatics, Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, Germany
Marcus Frohme
Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Hochschulring 1, 15745 Wildau, Germany; Corresponding author. Fax: +49 (0) 3375 508 261.
The presented dataset describes the quantification of carbon nanoparticle C60 fullerene accumulated in mitochondria of human leukemic cells treated with nanostructure. Firstly, the high performance liquid chromatography–electro spray ionization–mass spectrometry (HPLC-ESI-MS) method was developed for quantitative analysis of pristine C60 fullerene. Then, human leukemic cells were incubated with C60 fullerene, homogenized and subjected to the differential centrifugation to retrieve mitochondrial fraction. The C60 fullerene content was quantified by HPLC-ESI-MS in extracts of cellular fractions.This data article refers to the research article “C60 Fullerene Accumulation in Human Leukemic Cells and Perspectives of LED-mediated Photodynamic Therapy” by Grebinyk et al. [1].
