An efficient ELISA protocol for measurement of SARS-CoV-2 spike-specific IgG in human plasma and serum samples
Gwenllian A. Appeltrath,
Janine Parreuter,
Monika Lindemann,
Hannes Klump,
Christina B. Karsten
Affiliations
Gwenllian A. Appeltrath
Institute for the Research on HIV and AIDS-associated Diseases, University Hospital Essen, University of Duisburg-Essen, Essen, Germany
Janine Parreuter
Institute for the Research on HIV and AIDS-associated Diseases, University Hospital Essen, University of Duisburg-Essen, Essen, Germany
Monika Lindemann
Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany
Hannes Klump
Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany; Institute for Transfusion Medicine and Cell Therapeutics, University Hospital RWTH Aachen, Aachen, Germany
Christina B. Karsten
Institute for the Research on HIV and AIDS-associated Diseases, University Hospital Essen, University of Duisburg-Essen, Essen, Germany; Corresponding author.
Here, we describe a protocol for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike-specific immunoglobulin G (IgG) by enzyme-linked immunosorbent assay (ELISA). The protocol was developed with a keen focus on optimizing several key parameters, including antigen coating concentration, antibody and sample dilutions, and assay development time. The final protocol features the following characteristics: • The capability to detect SARS-CoV-2 spike-specific IgG in both plasma and serum samples. • A streamlined procedure that requires only 1 hour and 20 minutes of hands-on time. • Reliable assay performance, with a remarkable sensitivity of 98.1 % and specificity of 99.5 %.
