Isolation and Characterisation of Alongshan Virus in Russia
Ivan S. Kholodilov,
Alexander G. Litov,
Alexander S. Klimentov,
Oxana A. Belova,
Alexandra E. Polienko,
Nikolai A. Nikitin,
Alexey M. Shchetinin,
Anna Y. Ivannikova,
Lesley Bell-Sakyi,
Alexander S. Yakovlev,
Sergey V. Bugmyrin,
Liubov A. Bespyatova,
Larissa V. Gmyl,
Svetlana V. Luchinina,
Anatoly P. Gmyl,
Vladimir A. Gushchin,
Galina G. Karganova
Affiliations
Ivan S. Kholodilov
Laboratory of Biology of Arboviruses, Chumakov Institute of Poliomyelitis and Viral Encephalitides, FSBSI Chumakov FSC R&D IBP RAS, 108819 Moscow, Russia
Alexander G. Litov
Laboratory of Biology of Arboviruses, Chumakov Institute of Poliomyelitis and Viral Encephalitides, FSBSI Chumakov FSC R&D IBP RAS, 108819 Moscow, Russia
Alexander S. Klimentov
Laboratory of Biochemistry, Chumakov Institute of Poliomyelitis and Viral Encephalitides, FSBSI Chumakov FSC R&D IBP RAS, 108819 Moscow, Russia
Oxana A. Belova
Laboratory of Biology of Arboviruses, Chumakov Institute of Poliomyelitis and Viral Encephalitides, FSBSI Chumakov FSC R&D IBP RAS, 108819 Moscow, Russia
Alexandra E. Polienko
Laboratory of Biology of Arboviruses, Chumakov Institute of Poliomyelitis and Viral Encephalitides, FSBSI Chumakov FSC R&D IBP RAS, 108819 Moscow, Russia
Nikolai A. Nikitin
Faculty of Biology, Lomonosov MSU, 119991 Moscow, Russia
Alexey M. Shchetinin
Pathogenic Microorganisms Variability Laboratory, Gamaleya Federal Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, Russia
Anna Y. Ivannikova
Laboratory of Biology of Arboviruses, Chumakov Institute of Poliomyelitis and Viral Encephalitides, FSBSI Chumakov FSC R&D IBP RAS, 108819 Moscow, Russia
Lesley Bell-Sakyi
Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Liverpool L3 5RF, UK
Alexander S. Yakovlev
Laboratory of Biology of Arboviruses, Chumakov Institute of Poliomyelitis and Viral Encephalitides, FSBSI Chumakov FSC R&D IBP RAS, 108819 Moscow, Russia
Sergey V. Bugmyrin
Laboratory for Animal and Plant Parasitology, Institute of Biology of Karelian Research Centre, Russian Academy of Sciences (IB KarRC RAS), 185910 Petrozavodsk, Russia
Liubov A. Bespyatova
Laboratory for Animal and Plant Parasitology, Institute of Biology of Karelian Research Centre, Russian Academy of Sciences (IB KarRC RAS), 185910 Petrozavodsk, Russia
Larissa V. Gmyl
Laboratory of Biochemistry, Chumakov Institute of Poliomyelitis and Viral Encephalitides, FSBSI Chumakov FSC R&D IBP RAS, 108819 Moscow, Russia
Svetlana V. Luchinina
Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, 454092 Chelyabinsk, Russia
Anatoly P. Gmyl
Laboratory of Biology of Arboviruses, Chumakov Institute of Poliomyelitis and Viral Encephalitides, FSBSI Chumakov FSC R&D IBP RAS, 108819 Moscow, Russia
Vladimir A. Gushchin
Faculty of Biology, Lomonosov MSU, 119991 Moscow, Russia
Galina G. Karganova
Laboratory of Biology of Arboviruses, Chumakov Institute of Poliomyelitis and Viral Encephalitides, FSBSI Chumakov FSC R&D IBP RAS, 108819 Moscow, Russia
In recent decades, many new flavi-like viruses have been discovered predominantly in different invertebrates and, as was recently shown, some of them may cause disease in humans. The Jingmenvirus (JMV) group holds a special place among flaviviruses and flavi-like viruses because they have a segmented ssRNA(+) genome. We detected Alongshan virus (ALSV), which is a representative of the JMV group, in ten pools of adult Ixodes persulcatus ticks collected in two geographically-separated Russian regions. Three of the ten strains were isolated in the tick cell line IRE/CTVM19. One of the strains persisted in the IRE/CTVM19 cells without cytopathic effect for three years. Most ALSV virions purified from tick cells were spherical with a diameter of approximately 40.5 nm. In addition, we found smaller particles of approximately 13.1 nm in diameter. We obtained full genome sequences of all four segments of two of the isolated ALSV strains, and partial sequences of one segment from the third strain. Phylogenetic analysis on genome segment 2 of the JMV group clustered our novel strains with other ALSV strains. We found evidence for the existence of a novel upstream open reading frame in the glycoprotein-coding segment of ALSV and other members of the JMV group.
