<i>Purshia plicata</i> Triggers and Regulates Proteins Related to Apoptosis in HeLa Cancer Cells
Patricia Álvarez-Ortiz,
Juan Ascacio-Valdés,
Ileana Vera-Reyes,
Cecilia Esparza-González,
Raúl Rodríguez-Herrera,
Mauricio Salinas-Santander,
Mayela del Ángel-Martínez,
Antonio Morlett-Chávez
Affiliations
Patricia Álvarez-Ortiz
Laboratory of Molecular Biology, Chemistry School, Autonomous University of Coahuila, Saltillo 25280, Mexico
Juan Ascacio-Valdés
Bioprocesses and Bioproducts Research Group and Laboratory of Molecular Biology, Food Research Department, Chemistry School, Autonomous University of Coahuila, Saltillo 25280, Mexico
Ileana Vera-Reyes
Proteomics Laboratory, Agricultural Plant Science and Biotechnology, Research Center for Applied Chemistry, Blvd. Enrique Reyna 140, Saltillo 25294, Mexico
Cecilia Esparza-González
Laboratory of Histology, Dentistry School, Autonomous University of Coahuila, Saltillo 25125, Mexico
Raúl Rodríguez-Herrera
Bioprocesses and Bioproducts Research Group and Laboratory of Molecular Biology, Food Research Department, Chemistry School, Autonomous University of Coahuila, Saltillo 25280, Mexico
Mauricio Salinas-Santander
Laboratory of Molecular Biology, Health Research Department, Medicine School, Autonomous University of Coahuila, Saltillo 25000, Mexico
Mayela del Ángel-Martínez
Laboratory of Molecular Biology, Health Research Department, Medicine School, Autonomous University of Coahuila, Saltillo 25000, Mexico
Antonio Morlett-Chávez
Laboratory of Molecular Biology, Chemistry School, Autonomous University of Coahuila, Saltillo 25280, Mexico
Cervical cancer represents a public health problem, develops resistance to traditional therapies and cost-of-treatment is high. These disadvantages have led to the search for alternative bioactive-compound-based therapies. Said bioactive compounds include phenolic compounds, flavonoids, and tannins. The present study aimed to evaluate the therapeutic effect of a P. plicata extract on the HeLa cell line. Viability and apoptosis assays were run on the two cell lines treated with the extract. The peptides, up- and down-expressed in both cell lines, were identified by PDQuest analysis software and high-performance liquid chromatography/mass spectrometry/mass spectrometry (HPLC/MS/MS). Our results show that a 500 mg/L treatment deregulated cell viability, with different apoptotic morphologies observed which are associated with the presence of bio-compounds, which up- and down-regulated the peptides. In conclusion, P. plicata regulates proteins associated with apoptosis in HeLa cancer cells.
