THE CORRESPONDENCE OF THE CARBAPENEMASE GENOTYPE AND PHENOTYPIC ANTIMICROBIAL PROFILES OF PSEUDOMONAS AERUGINOSA

Abstract

The aim of the study was to determine the correspondence between the carbapenemase genotype and the phenotypic antimicrobial profiles of P. aeruginosa. Materials and methods. The study included 51 clinical isolates of P. aeruginosa, isolated from the patients with post-operative complications of the respiratory organs. The final identification of the obtained isolates was performed in the Riesbeck laboratory using MALDI-ToF (Bruker), followed by the determination of their sensitivity to antimicrobial drugs at the EUCAST Development Laboratory (Växjö, Sweden). Determination of the resistance genes was carried out by using polymerase chain reaction in real time (PCR-RF). The antimicrobial resistance index (ARI) was determined according to the method of G.V. de Socio. Statistical analysis was performed using the standard IBM SPSS Statistics software version 22.0 and GraphPad Prism Software 10.1.0. (USA, 2023). Results. 39 strains of P. aeruginosa (76.5%) showed polyresistance, and 26 of them (51.0%) were resistant to all antibiotics. According to research data, P. aeruginosa isolates most often carried the blaVIM gene. Genetically determined production of oxacillinase group -lactamase class D among clinical isolates of P. aeruginosa occurred somewhat less often. Based on the obtained results, four carbapenemase genetic resistotypes of P. aeruginosa as pathogens of respiratory tract complications in critically ill patients were established. We detected the antimicrobial resistance index (ARI) based on the phenotypic characteristics of P. aeruginosa at the level of 0.690.39. The phenomenon of statistically reliable correlation of the ARI of microorganisms by phenotypic characteristics with their carbapenemase genetic resistotypes was established. Conclusions. 76.5% of strains of P. aeruginosa show polyresistance, and 51.0% of them are resistant to all antibiotics. Four different carbapenemase genetic resistotypes of P. aeruginosa as pathogens of respiratory tract complications in critically ill patients were established. There is the phenomenon of statistically reliable correlation of the ARI of microorganisms by phenotypic characteristics with their carbapenemase genetic resistotypes.

Keywords

• p. aeruginosa
• antibiotics
• drug resistance
• multi-drug resistance
• carbapenems
• phenotypic profile
• genes of resistance