Nature Communications (May 2019)

SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation

  • Takuya Kuroda,
  • Satoshi Yasuda,
  • Shiori Tachi,
  • Satoko Matsuyama,
  • Shinji Kusakawa,
  • Keiko Tano,
  • Takumi Miura,
  • Akifumi Matsuyama,
  • Yoji Sato

DOI
https://doi.org/10.1038/s41467-019-09511-4
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 13

Abstract

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Human induced pluripotent stem cells (hiPSCs) generate all cell types in the body, but different lines can differ in their potential. Here, the authors determine that higher endogenous levels of SALL3 in hiPSCs lead to ectoderm differentiation bias and reduced mesoderm/endoderm due to DNMT3B mediated DNA methylation.