Engineering and optimising deaminase fusions for genome editing

Luhan Yang; Adrian W. Briggs; Wei Leong Chew; Prashant Mali; Marc Guell; John Aach; Daniel Bryan Goodman; David Cox; Yinan Kan; Emal Lesha; Venkataramanan Soundararajan; Feng Zhang; George Church

doi:10.1038/ncomms13330

Nature Communications (Nov 2016)

Engineering and optimising deaminase fusions for genome editing

Luhan Yang,
Adrian W. Briggs,
Wei Leong Chew,
Prashant Mali,
Marc Guell,
John Aach,
Daniel Bryan Goodman,
David Cox,
Yinan Kan,
Emal Lesha,
Venkataramanan Soundararajan,
Feng Zhang,
George Church

Affiliations

Luhan Yang: Department of Genetics, Harvard Medical School
Adrian W. Briggs: Department of Genetics, Harvard Medical School
Wei Leong Chew: Department of Genetics, Harvard Medical School
Prashant Mali: Department of Genetics, Harvard Medical School
Marc Guell: Department of Genetics, Harvard Medical School
John Aach: Department of Genetics, Harvard Medical School
Daniel Bryan Goodman: Department of Genetics, Harvard Medical School
David Cox: Harvard-MIT Division of Health Science and Technology
Yinan Kan: Department of Genetics, Harvard Medical School
Emal Lesha: Department of Genetics, Harvard Medical School
Venkataramanan Soundararajan: Department of Genetics, Harvard Medical School
Feng Zhang: Broad Institute of MIT and Harvard
George Church: Department of Genetics, Harvard Medical School

DOI: https://doi.org/10.1038/ncomms13330
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 12

Abstract

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Precision genome engineering using homology donors and the endogenous DNA break repair machinery and recently CRISPR-Cas9 targeted APOBECs have been demonstrated. Here the authors design zinc-finger and TALE chimeric deaminases and investigate editing efficiency and off-target effects.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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