Cancer Management and Research (Aug 2020)

Long Noncoding RNA LINC00173 Promotes NUTF2 Expression Through Sponging miR-765 and Facilitates Tumorigenesis in Glioma

  • Du Q,
  • Liu J,
  • Tian D,
  • Zhang X,
  • Zhu J,
  • Qiu W,
  • Wu J

Journal volume & issue
Vol. Volume 12
pp. 7211 – 7217

Abstract

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Qinghua Du,1,* Jin Liu,1,* Da Tian,1 Xuelei Zhang,1 Jinwei Zhu,1 Weiwen Qiu,2 Jun Wu3 1Neurosurgery Department, Lishui City People’s Hospital, Lishui 323000, People’s Republic of China; 2Neurology Department, Lishui City People’s Hospital, Lishui 323000, People’s Republic of China; 3Pathology Department, Lishui City People’s Hospital, Lishui 323000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jun Wu; Weiwen Qiu Email [email protected]; [email protected]: Glioma is one of the leading causes of cancer-related deaths. This study aimed to investigate the function and mechanism of long noncoding RNA (lncRNA) LINC00173 in the regulation of glioma progression.Methods: LINC00173 expression was measured using qRT-PCR. Survival rate was analyzed through Kaplan–Meier method. CCK8, colony formation and EdU assays were performed to measure cell proliferation while transwell was used to determine cell migration and invasion. Luciferase reporter assay was conducted to test RNA interaction.Results: LINC00173 expression was elevated in glioma tissues and cells. LINC00173 high expression predicted poor prognosis. Loss of LINC00173 inhibited proliferation, migration and invasion. LINC00173 interacted with miR-765 to enhance NUTF2 expression. MiR-765 expression was negatively correlated with LINC00173 and NUTF2 in glioma tissues. NUTF2 level was increased in glioma tissues. NUTF2 overexpression rescued the potential of proliferation, migration and invasion in LINC00173-silenced cells.Conclusion: Our research demonstrated that LINC00173 promotes glioma progression through targeting miR-765/NUTF2 axis.Keywords: LINC00173, miR-765, NUTF2, glioma

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