Química Nova (Oct 2019)

HDL-PARTICLES SEPARATION EMPLOYING DIFFERENT PRECIPITATING AGENTS: FUNCTIONAL PROPERTIES OF THE IMPACT OF CHEMICAL PRECIPITATION ON LIPOPROTEIN PARTICLE-SIZE AND PARAOXONASE-1-ACTIVITY

  • Vanessa R. A. e Silva,
  • Elaine C. Albuquerque,
  • Ana Paula C. dos Santos,
  • Julio C. A. Santos,
  • Lazaro S. S. Junior,
  • William A. Presada,
  • Carmen G. C. de M. Vinagre,
  • Ricardo D. Couto

DOI
https://doi.org/10.21577/0100-4042.20170410
Journal volume & issue
Vol. 42, no. 8
pp. 859 – 865

Abstract

Read online Read online

First-generation methods used to quantify HDL-cholesterol have been used for decades. Therefore, first-generation precipitatingreagents still in use, the chemical impact on HDL-particles functionality (structure/activity-relationship) is not well-documented. The study aim was to identify the most efficient precipitating-reagent for lipoprotein separation to be used in the HDL functionalevaluation. A cross-sectional design with convenience sampling was conducted. Plasma samples from 40 individuals, both genders, were evaluated. Biochemical analysis was performed after using different chemical precipitants (e.g. polyethylene glycol - PEG (P1), phosphotungstic-acid (P2), and dextran-sulfate/MgCl2 (P3)) to evaluate HDL-particle-size and PON1-activity. Parametric and non-parametric tests were performed using GraphPad-Prism 5.01 (USA). Differences were considered significant when a p-value < 0.05 were obtained. The PON1-activity comparison differed after NaCl stimulation (p < 0.001, paired t-test) and among chemical precipitant treatments. The PON1-activity differed after sample precipitation with P1 when compared to P2, and P3 (p < 0.05; ANOVA), before and after stratification for dyslipidemia group. The HDL particle-size comparison did not differ, but on the other hand, polydispersity differed after sample precipitation with P3 when compared to P1 or P2 (p < 0.05; ANOVA). Dextran- Sulfate/MgCl2 was a better chemical precipitant for PON1-activity, and PEG for HDL-particle-size, respectively. The HDL functional

Keywords