Basic & Clinical Cancer Research (Jan 2022)
hsa_circ_0001518 as a Diagnostic and Targeted Therapy Biomarker in Early Diagnosis and Treatment of Breast Cancer
Abstract
Background and Goal:After lung cancer, breast cancer is considered as the second prevailed type of cancer among women. Circular RNAs are a group of non-coding RNAs that through endogenous RNAs’ mechanism play role in tumorigenesis and progression of malignancies. However, little information is known about their role and importance in cancer progression and their chemical resistance. The research has been performed to the aim of studying effect of effective dosages of Iron superoxide and nickel oxide nanoparticles, and Q10 antioxidant alone or simultaneously on hsa_circ_0001518 expression in mice with breast cancer compared to healthy mice. Materials and Methods: In this experimental study, 120 mature female mice BALB/c (Five groups of 10 mice each) have been studied in two groups of healthy mice and those with breast cancer. Inducing breast cancer has been taken place through injection of 4T1 cell line to mice. IC50has been specified on 4T1 cell line through 48 hours treatment with iron superoxide (50, 100, 150, and 200mcg/ml) and nickel oxide (10, 20, 30, and 40mcg/ml) nanoparticles, and Q10 antioxidant (20, 60, 80, and 100mcg/ml). Finally, effect of IC50in treatments alone and combination therapy with iron superoxide, nickel oxide, and Q10 antioxidant on hsa_circ_0001518 has been evaluated, using real-time-PCR. Findings: The results from bioinformatic analysis of circBase showed that hsa_circ_0001518 affects Bcl2 apoptosis inhibitor gene and can play role in creation and progression of breast cancer through anti-apoptotic effects. IC50has been calculated to be respectively equal to 42.92, 49.21, and 47.83mcg/ml for nanoparticles of iron superoxide, nickel oxide, and Q10 antioxidant. The results related for real time PCR showed that expression level of hsa_circ_0001518 under combination therapy with nanoparticles of iron superoxide, nickel oxide and Q10 antioxidant in cancerous cells compared to healthy cells has shown significant reduction. Discussion and Conclusion:The research results confirm increase of cell damage resulted from oxidative stress of nanoparticles of iron superoxide and nickel oxide in combination therapy with Q10 antioxidant compared to treatments alone in cancerous mice. So, using nanoparticles and Q10 simultaneously can be considered in designing a medicine for breast cancer treatment. Oxidative stress resulted from nanoparticle treatment in combination with Q10 can have inhibitory effect on Q10 antioxidant properties in cancerous cells; and, with induction of apoptosis; it may lead to decrease of hsa_circ_0001518 expression. Therefore, studying changes of expression level of hsa_circ_0001518 can be taken into consideration in future and after performance of follow up studies as a molecular biomarker in breast cancer diagnosis and target therapy.
