Synthesis, characterization and biological activities of imidazo[1,2-a]pyridine based gold(III) metal complexes

Abstract

Five imidazo [1,2-a]pyridine derivatives and their Au(III) complexes were synthesized. The compounds were characterized by 1H-NMR, 13C-NMR, IR, mass, UV-visible, elemental analysis, conductivity and magnetic measurement studies. All the compounds were screened for diverse biological activities to check the effect of coordination of Au(III) with imidazo [1,2-a]pyridine heterocycles. The DNA interaction ability of compounds were studied as the change in absorption maxima and position of HS-DNA in presence of compounds and viscosity measurement due to change in DNA length under the influence of compounds. The computational insight of compound-DNA interaction was taken in docking study. All the results suggest intercalation mode of binding. The cellular level cytotoxic nature of compounds was evaluated using trypan blue dye staining of dead cell in cell viability assay. The smearing of DNA was observed, while DNA extracted from S. pombe cells in presence of complexes was subjected to gel electrophoresis, which shows their toxic effect on DNA. The complexes were evaluated for cytotoxicity on human A549 (Lung adenocarcinoma) cell line by MTT assay (IC50 values). The in vitro cytotoxicity in terms of LC50 value was checked on a simple zoological organism, brine shrimp.

Keywords

• Inorganic chemistry
• Materials chemistry
• Organic chemistry
• Pharmaceutical chemistry
• Imidazo[1,2-a]pyridine
• A549 (Lung adenocarcinoma) cell line