Immunomodulatory effect of Acanthopanax senticosus polysaccharide on immunosuppressed chickens

Shubao Yang; Chunlan Shan; Xin Ma; Yanjun Qin; Anqi Ju; Aoyi Duan; Weimin Luan; Yingnan Zhang

Poultry Science (Feb 2021)

Immunomodulatory effect of Acanthopanax senticosus polysaccharide on immunosuppressed chickens

Shubao Yang,
Chunlan Shan,
Xin Ma,
Yanjun Qin,
Anqi Ju,
Aoyi Duan,
Weimin Luan,
Yingnan Zhang

Affiliations

Shubao Yang: Center For Reproductive Medicine, Jilin Medical University, Jilin 132013, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China
Chunlan Shan: College of Animal Science and Technology, Yunnan Agricultural University, Yunnan 650210, China
Xin Ma: College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China
Yanjun Qin: College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China
Anqi Ju: College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China
Aoyi Duan: College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China
Weimin Luan: College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China
Yingnan Zhang: School of Public Health, Jilin Medical University, Jilin 132013, China; College of Life Science, Changchun Sci-Tech University, Changchun 130600, China; Corresponding author:

Journal volume & issue: Vol. 100, no. 2
pp. 623 – 630

Abstract

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The immunomodulatory effect of Acanthopanax senticosus polysaccharide (ASPS) on immunosuppressed chickens induced by cyclophosphamide (Cy) was observed in this study. Four hundred 7-day-old chickens were randomly divided into 4 groups: vaccinated control group (VC group), Cy-challenged control group (Cy group), Cy-challenged + low-dose ASPS group (ASPSL + Cy group), and Cy-challenged + high-dose ASPS group (ASPSH + Cy group). All groups except the VC group were injected with Cy at a dose of 80 mg/kg/day of BW for 3 successive days to induce immunosuppression. At the age of 10 d, the ASPSL + Cy group and ASPSH + Cy group were intramuscularly injected with 0.2 mL of ASPS at the dose of 100 and 200 mg/mL/day, respectively, once a day for 3 successive days. The Cy group was injected with saline solution in the same way as the 2 ASPS groups. At the age of 14 d, the chickens were vaccinated with Newcastle disease (ND) vaccine in all groups. On day 7, 14, 21, and 28 after the vaccination, BW, lymphocyte proliferation, the serum antibody titers of the ND vaccine, the proportion of CD4+ and CD8+ T lymphocytes, and the concentrations of interferon gamma and IL-2 were determined. The results showed that chickens were injected with Cy at a dose of 80 mg/kg of BW for 3 d displayed lower immune responses than the control group, indicating that the immunosuppressive model was successfully established. At most time points, both high and low doses of ASPS could significantly promote lymphocyte proliferation; enhance BW, antibody titers, and the proportion of CD4+ and CD8+ T lymphocytes; and raised the concentrations of interferon gamma and IL-2 in Cy-treated chickens compared with those in the Cy control group (P < 0.05). These results indicated that ASPS could resist immunosuppression induced by Cy and may be a new-type immune adjuvant to improve vaccination in normal and immunosuppressed chickens.

Published in Poultry Science

ISSN: 0032-5791 (Print); 1525-3171 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Agriculture: Animal culture
Website: https://www.journals.elsevier.com/poultry-science

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