Cyprinid Herpesvirus 2 miR-C12 Attenuates Virus-Mediated Apoptosis and Promotes Virus Propagation by Targeting Caspase 8

Jianfei Lu; Jianfei Lu; Jianfei Lu; Zhaoyuan Shen; Zhaoyuan Shen; Zhaoyuan Shen; Liqun Lu; Liqun Lu; Liqun Lu; Dan Xu; Dan Xu; Dan Xu; Dan Xu

doi:10.3389/fmicb.2019.02923

Frontiers in Microbiology (Dec 2019)

Cyprinid Herpesvirus 2 miR-C12 Attenuates Virus-Mediated Apoptosis and Promotes Virus Propagation by Targeting Caspase 8

Jianfei Lu,
Jianfei Lu,
Jianfei Lu,
Zhaoyuan Shen,
Zhaoyuan Shen,
Zhaoyuan Shen,
Liqun Lu,
Liqun Lu,
Liqun Lu,
Dan Xu,
Dan Xu,
Dan Xu,
Dan Xu

Affiliations

Jianfei Lu: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo, China
Jianfei Lu: Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo, China
Jianfei Lu: Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo, China
Zhaoyuan Shen: National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, China
Zhaoyuan Shen: Key Laboratory of Aquaculture Ministry for Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Shanghai, China
Zhaoyuan Shen: National Experimental Teaching Demonstration Center for Fishery Sciences, Shanghai Ocean University, Shanghai, China
Liqun Lu: National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, China
Liqun Lu: Key Laboratory of Aquaculture Ministry for Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Shanghai, China
Liqun Lu: National Experimental Teaching Demonstration Center for Fishery Sciences, Shanghai Ocean University, Shanghai, China
Dan Xu: National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, China
Dan Xu: Key Laboratory of Aquaculture Ministry for Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Shanghai, China
Dan Xu: National Experimental Teaching Demonstration Center for Fishery Sciences, Shanghai Ocean University, Shanghai, China
Dan Xu: Guangxi Key Laboratory for Marine Biotechnology, Guangxi Institute of Oceanography, Guangxi Academy of Sciences, Beihai, China

DOI: https://doi.org/10.3389/fmicb.2019.02923
Journal volume & issue: Vol. 10

Abstract

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DNA viruses, most notably members of the herpesvirus family, generally encode miRNAs to mediate both virus and host genes expression. We previously demonstrated that Cyprinid herpesvirus 2 (CyHV-2) encodes 17 miRNAs that are involved in innate immune signaling pathways. In this study, the function of CyHV-2-encoded miRNA was further investigated in GiCF cells. We found that miR-C4 promoted CyHV-2-induced apoptosis, while miR-C12 decreased CyHV-2-induced apoptosis. miR-C12 targeted to 3′ UTR sequence of caspase 8 and suppressed the expression of caspase 8. Besides, the silencing of caspase 8 by specific siRNA led to the attenuation of CyHV-2-induced apoptosis. Furthermore, caspase 8 was downregulated in cells transfected with miR-C12 during CyHV-2 infection. Overexpression of miR-C12 significantly suppressed CyHV-2-induced apoptosis, while silencing of miR-C12 promoted CyHV-2-induced apoptosis. Finally, inhibition of miR-C12 resulted in suppression of CyHV-2 propagation, overexpression of miR-C12, and CASP8-siRNA-1 facilitated CyHV-2 propagation. Taken together, our results demonstrated that CyHV-2-encoded miR-C12 to suppress virus-induced apoptosis and promoted virus replication by targeting caspase 8.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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