Proteomics dataset containing proteins that obscure identification of TOPLESS interactors in Arabidopsis
Joe Collins,
Craig Dufresne,
William B. Gurley,
Sixue Chen
Affiliations
Joe Collins
Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL, USA; Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL, USA
Craig Dufresne
Thermo Fisher Scientific, West Palm Beach, FL, USA
William B. Gurley
Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL, USA; Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
Sixue Chen
Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL, USA; Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL, USA; Proteomics and Mass Spectrometry, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL, USA; Corresponding author at: Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL, USA
Here we report proteins identified after conducting Tandem Affinity Purification (TAP) of the TOPLESS (TPL) corepressor from Arabidopsis. We generated transgenic plants harboring TPL fused to the GS-TAG, “Boosting tandem affinity purification of plant protein complexes” (Van Leene et al., 2008) [1]. Four independent biological replicates of a selected TPL-GS-TAG line were grown simultaneously, crosslinked with formaldehyde, and proteins were isolated from whole plant tissue via TAP. Purified proteins were treated with trypsin, and the peptides were analyzed via mass spectrometry. Datasets are hosted in the MassIVE public repository (reference number: MSV000082477, https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=f16255fb7080426a9fe1926b4d3d5862). The data in this article has not been published elsewhere and is original to this work.
