Comparative Study of Biofilm Formation in Pseudomonas aeruginosa Isolates from Patients of Lower Respiratory Tract Infection

Abstract

Background: This study assessed biofilm formations of P.aeruginosa which was isolated from patients with Lower Respiratory Tract Infections (LRTIs). Objective: This study was conducted to compare different methods of biofilm formations seen in P. aeruginosa which was obtained from LRTI patients. Materials and Methods: In this cross-sectional study, we investigated a total of 80 P. aeruginosa isolates obtained from LRTI patients by different methods. Tube method (TM), tissue culture plate (TCP) method and modified tissue culture plate (MTCP) method. They were subjected to biofilm detection methods. Results: The MTCP method produced a higher accuracy ratio than TCP method. In terms of sensitivity and specificity, the MTCP method was considered to be superior to TM. We observed a higher antibiotic resistance in biofilm producing bacteria than in non-biofilm producers. Conclusion: In our study, MTCP was found to be more sensitive and specific method for biofilm detection than TCP and TM.

Keywords

• biofilm formation
• p. aeruginosa
• tissue culture plate
• lower respiratory tract infection