The Effect of Repaglinide on Polycystic Ovary Induced by Testosterone Enanthate in NMRI Mice

Abstract

Background & Objective: Poly Cystic Ovarian Syndrome (PCOS) is the most common cause of infertility due to anovulation in women. One of the treatments of this syndrome is using Insulin sensitive drugs. The main objective of this project is to analyze the effect of Repaglinide on the histomorphology of polycystic ovary.  Materials & Methods: In this study, PCOS is induced by the injection of Testosterone Enanthate in immature mice aged five to seven weeks old for four weeks. Then, the mice were divided in to two groups of control and case. The control group had no injection. In the case group, each mouse received 30 µgr Repaglinide. Body weight, the ratio of ovary weight to body weight, and ovarian diameter were measured and the changes in histomorphology of the ovary, characteristics of ovarian tissue, and the number of follicles were investigated. T- Test was used in this study (T-test, P£0.05. ( Results: The result revealed that Repaglinide had a reducing effect on body weight, the ratio of ovary weight to body weight, and the diameter of ovary (P£0.05). Repaglinide also caused a decrease in Tunica albuginea changes, hyperthecosis, the number of degenerated oocyte, granolusa cell, picnosis and hyper vascularization. The average of primordial, primary, pre-antral, cystic, and atretic follicles are significantly decreased in the case group compared to the control group (P£0.05). Conclusion: Repaglinide can compensate the PCOS ovarian damages. Further studies are recommended in this field.

Keywords

• polycystic ovary
• repaglinide
• diabetes
• fertility