Multi-omics dissection of metabolic and transcriptional regulation underlying fruit maturation in Panax ginseng

Abstract

Abstract Background Panax ginseng is a perennial plant valued for its medicinal and nutritional properties. Its fruit contains a variety of bioactive compounds such as ginsenosides, flavonoids, phenolic acids, and anthocyanins. However, the regulatory mechanisms underlying the accumulation of these compounds during fruit development remain largely unexplored. Results We performed integrated metabolomic and transcriptomic analyses across four developmental stages of ginseng fruit. Metabolite profiling revealed stage-specific accumulation patterns of ginsenosides and phenolics with biphasic trends, and increasing levels of flavonoids and anthocyanins during maturation. We constructed a metabolic and gene expression atlas covering primary metabolism (carbon, amino acids, nitrogen), secondary metabolism (flavonoids, terpenoids), and hormone signaling pathways (abscisic acid, gibberellin, brassinosteroids). Key structural genes and transcription factors, including MYB, bHLH, and ERF families, were found to coordinate stage-specific metabolic shifts. Weighted gene co-expression network analysis revealed metabolite-linked gene modules that delineate regulatory relationships. Conclusions This study provides a comprehensive molecular framework of fruit development in P. ginseng, highlighting coordinated transcriptional regulation and metabolic reprogramming. These insights contribute to our understanding of developmental regulation in medicinal plants and lay the groundwork for metabolic engineering strategies aimed at enhancing nutritional quality and bioactive compound production.

Keywords

• Panax ginseng
• Multi-omics integration
• Fruit development
• Secondary metabolism
• Primary metabolism
• Transcription factors