Optimizing start-up strategies for the two-inflow nitritation/anammox process: Influence on biofilm microbial community composition

Abstract

Low-energy nitrogen removal from ammonium-rich wastewater is crucial in preserving the water environment. A one-stage nitritation/anammox process with two inflows treating ammonium-containing wastewater, supplied from inside and outside the wound filter, is expected to stably remove nitrogen. Laboratory-scale reactors were operated using different start-up strategies; the first involved adding nitritation inoculum after anammox biomass formation in the filter, which presented a relatively low nitrogen removal rate (0.171 kg N/m3 · d), at a nitrogen loading rate of 1.0 kg N/m3 · d. Conversely, the second involved the gradual cultivation of anammox and nitritation microorganisms, which increased the nitrogen removal rate (0.276 kg N/m3 · d). Furthermore, anammox (Candidatus Brocadia) and nitritation bacteria (Nitrosomonadaceae) coexisted in the biofilm formed on the filter surface. The abundance of nitritation bacteria (10.5%) in the reactor biofilm using the second start-up strategy was higher than that using the first (3.7%). Thus, the two-inflow nitritation/anammox process effectively induced habitat segregation using a suitable start-up strategy. HIGHLIGHTS The addition of nitritation inoculum after the anammox biofilm formation in the two-inflow partial nitritation/anammox (PNA) process does not increase nitrogen removal.; Gradual cultivation of anammox and nitritation microorganisms leads to better nitrogen removal.; The two-inflow PNA process effectively induced habitat segregation and Candidatus Brocadia and Nitrosomonadaceae formed a thick biofilm on the filter.;

Keywords

• biofilm
• candidatus brocadia
• habitat segregation
• nitrosomonadaceae
• one-stage nitritation/anammox process