Curcumin ameliorates H2O2-induced injury through SIRT1-PERK-CHOP pathway in pancreatic beta cells

Cao Zhaohui; Wu Zhuan; Duan Tingting; Tang Cifei; Huang Di; Hu Xiaobo

doi:10.3724/abbs.2022004

Acta Biochimica et Biophysica Sinica (Feb 2022)

Curcumin ameliorates H2O2-induced injury through SIRT1-PERK-CHOP pathway in pancreatic beta cells

Cao Zhaohui,
Wu Zhuan,
Duan Tingting,
Tang Cifei,
Huang Di,
Hu Xiaobo

Affiliations

Cao Zhaohui: ["Department of Biochemistry and Molecular Biology, Hengyang Medical School, University of South China, Hengyang 421001, China","Key Laboratory of Ecological Environment and Critical Human Diseases Prevention of Hunan Province Department of Education, Hengyang Medical School, University of South China, Hengyang 421001, China"]
Wu Zhuan: ["Department of Biochemistry and Molecular Biology, Hengyang Medical School, University of South China, Hengyang 421001, China","Key Laboratory of Ecological Environment and Critical Human Diseases Prevention of Hunan Province Department of Education, Hengyang Medical School, University of South China, Hengyang 421001, China","Central Laboratory, Guangxi Health Commission Key Laboratory of Glucose and Lipid Metabolism Disorders, the Second Affiliated Hospital of Guilin Medical University, Guilin 541000, China"]
Duan Tingting: ["Department of Biochemistry and Molecular Biology, Hengyang Medical School, University of South China, Hengyang 421001, China","Key Laboratory of Ecological Environment and Critical Human Diseases Prevention of Hunan Province Department of Education, Hengyang Medical School, University of South China, Hengyang 421001, China"]
Tang Cifei: ["Department of Biochemistry and Molecular Biology, Hengyang Medical School, University of South China, Hengyang 421001, China","Key Laboratory of Ecological Environment and Critical Human Diseases Prevention of Hunan Province Department of Education, Hengyang Medical School, University of South China, Hengyang 421001, China"]
Huang Di: ["Department of Biochemistry and Molecular Biology, Hengyang Medical School, University of South China, Hengyang 421001, China","Key Laboratory of Ecological Environment and Critical Human Diseases Prevention of Hunan Province Department of Education, Hengyang Medical School, University of South China, Hengyang 421001, China"]
Hu Xiaobo: ["Department of Biochemistry and Molecular Biology, Hengyang Medical School, University of South China, Hengyang 421001, China","Key Laboratory of Ecological Environment and Critical Human Diseases Prevention of Hunan Province Department of Education, Hengyang Medical School, University of South China, Hengyang 421001, China"]

DOI: https://doi.org/10.3724/abbs.2022004
Journal volume & issue: Vol. 54
pp. 370 – 377

Abstract

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Oxidative stress and endoplasmic reticulum (ER) stress play crucial roles in pancreatic β cell destruction, leading to the development and progression of type 1 diabetes mellitus (T1DM). Curcumin, extracted from plant turmeric, possesses multiple bioactivities such as antioxidant, anti-inflammatory and anti-apoptosis properties in vitro and in vivo. However, it remains unknown whether curcumin improves ER stress to prevent β cells from apoptosis. In this study, we aim to investigate the role and mechanism of curcumin in ameliorating H2O2-induced injury in MIN6 (a mouse insulinoma cell line) cells. Cell viability is examined by CCK8 assay. Hoechst 33258 staining, TUNEL and flow cytometric assay are performed to detect cell apoptosis. The relative amounts of reactive oxygen species (ROS) are measured by DCFH-DA. WST-8 is used to determine the total superoxide dismutase (SOD) activity. Protein expressions are determined by western blot analysis and immunofluorescence staining. Pretreatment with curcumin prevents MIN6 cells from H2O2-induced cell apoptosis. Curcumin decreases ROS generation and inhibits protein kinase like ER kinase (PERK)-C/EBP homologous protein (CHOP) signaling axis, one of the critical branches of ER stress pathway. Moreover, incubation with curcumin activates silent information regulator 1 (SIRT1) expression and subsequently decreases the expression of CHOP. Additionally, EX527, a specific inhibitor of SIRT1, blocks the protective effect of curcumin on MIN6 cells exposed to H2O2. In sum, curcumin inhibits the PERK-CHOP pathway of ER stress mediated by SIRT1 and thus ameliorates H2O2-induced MIN6 cell apoptosis, suggesting that curcumin and SIRT1 may provide a potential therapeutic approach for T1DM.

Published in Acta Biochimica et Biophysica Sinica

ISSN: 1672-9145 (Print); 1745-7270 (Online)
Publisher: China Science Publishing & Media Ltd.
Country of publisher: China
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry; Science: Biology (General): Genetics
Website: https://www.sciengine.com/ABBS/home

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