Hypersensitive Electrochemical Sequential Monitoring of Total IgE and their Effects with Ovalbumin Induced Mice

Abstract

Monitoring of specific biomarker is critical for determining progression of a disease or efficacy of a treatment. Currently, the standard for assessing amount of specific biomarkers is the enzyme linked immunosorbent assay (ELISA), which measures quantities on the nanogram scale. However, ELISA has high material costs, long incubation periods, requires large volume of samples and involves special instruments, which necessitates clinical samples to be sent to a lab. In order to provide rapid, accurate, easy, point-of-care measurement of biomarkers, electrochemical immunosensor can be used to provide specific and sensitive biomarker detection. Immunosensor allow near real-time results, reduced costs, and simple assays with no labelling. In this work, we developed an electrochemical biosensor to measure total immunoglobulin E (IgE), a marker of asthma disease that varies with age, gender, and disease in concentrations from 0.3–1000 ng/mL with consuming 20 µL volume of real blood sample. Also, sequential monitoring of total IgE with OVA induced mice is another application of this work and this sensor is an alternative approach for recording data and a more effective assay for understanding the cytotoxic effects of toxic materials.

Keywords

• electrochemical sensor
• sequential monitoring
• amperometric detection
• surface modification