Sequencing Analysis of cvaC Gene in Acinetobacter Baumannii That Isolates from Different Infections

Abstract

Acinetobacter baumannii is one of the opportunistic nurses responsible for many acquired infections in hospitals due to its ability to resist many antibiotics. This is one of the problems facing hospitals in the world. Identification of the cvaC gene and sequence analysis in Acinetobacter baumannii isolates from various infections, and mutation detection in this gene. From 1st of September to 30th of November, 2016, 200 Acinetobacter baumannii isolates were obtained from various clinical samples. Follow fifty isolates from blood, twenty isolates from urinary tract infections, thirty isolates from wound infections, forty isolates from burn infections and twenty-five isolates from stool samples from various hospitals (Central Children's Hospital, Al Karama Hospital, Karkh General Hospital, Al-Ameen Medical City Hospital, Educational Labs, Baghdad Teaching Hospital, Child Protection Hospital, Burns and Wounds Hospital) in Baghdad city. Identification forty isolates confirmed that they belong to Acinetobacter baumannii, including fourteen isolates from a stool sample, nine isolates from blood, eight isolates from burns, four isolates from wound infections, and Respiratory tract infection, , and only one isolate from urinary tract infections sample. Genotypic detection of the cvaC gene of Acinetobacter baumannii showed the presence of this gene in 16 isolates (40%) and Sequencing analysis of cvaC has shown seven genetic mutations and only one mutation has been converted amino acid Alanine to Valine.The amino acid Alanine was changed to Valine in Position 656 at Subject 678, resulting in silent mutations that did not affect protein translation and other mutations that resulted in a change in the amino acid arrangement and protein translation.

Keywords

• colicin
• genotypic analysis
• mutations