Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis

Chia-Lung Wu; Amanda Dicks; Nancy Steward; Ruhang Tang; Dakota B. Katz; Yun-Rak Choi; Farshid Guilak

doi:10.1038/s41467-020-20598-y

Nature Communications (Jan 2021)

Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis

Chia-Lung Wu,
Amanda Dicks,
Nancy Steward,
Ruhang Tang,
Dakota B. Katz,
Yun-Rak Choi,
Farshid Guilak

Affiliations

Chia-Lung Wu: Dept. of Orthopaedic Surgery, Washington University in Saint Louis
Amanda Dicks: Dept. of Orthopaedic Surgery, Washington University in Saint Louis
Nancy Steward: Dept. of Orthopaedic Surgery, Washington University in Saint Louis
Ruhang Tang: Dept. of Orthopaedic Surgery, Washington University in Saint Louis
Dakota B. Katz: Dept. of Orthopaedic Surgery, Washington University in Saint Louis
Yun-Rak Choi: Dept. of Orthopaedic Surgery, Washington University in Saint Louis
Farshid Guilak: Dept. of Orthopaedic Surgery, Washington University in Saint Louis

DOI: https://doi.org/10.1038/s41467-020-20598-y
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 18

Abstract

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Application of human induced pluripotent stem cells (hiPSCs) for tissue regeneration is hindered by off-target cell differentiation. Here, the authors use bulk and single cell RNA-sequencing to identify WNT and MITF as off-target hubs during chondrogenic differentiation; inhibiting these pathways enhanced homogeneity and yield.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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