Мать и дитя в Кузбассе (Mar 2019)
REGULATORY TRANSPORT PROTEINS AND CYTOKINES OF BLOOD SERUM IN THE PROGRAMS OF EXTRACORPORAL FERTILIZATION IN WOMEN WITH MALE INFERTILITY FACTOR
Abstract
Objective of research – studies of content and IVF programs outcome impact of certain regulatory and transport proteins: alfa2-macroglobulin (α2-MG), alfa1-antitrypsin (α1-АТ), pregnancy associated alfa2-glycoprotein (PAG), lactoferrin (LF), albumin (ALB), total protein (TP) and cytokines: tumor necrosis factor-alfa (TNF-α), interleukins 6 and 8 (IL-6 and IL-8), interferon gamma (INF-γ), vascular endothelial growth factor VEGF in blood serum of women with male factor infertility. Materials and methods. Examination was performed of 37 infertile patients with male genesis infertility (out of whom 20 – with primary infertility and 17 – secondary). Parameters under research prior to IVF program entry were compared against the data of 33 healthy fertile non-pregnant women. Infertile patients underwent IVF/ICSI program, resulting in pregnancy occurrence in 14 of them (38 %) and non-occurrence in 23 (62 %). Contents of α2-MG, α1-АТ, PAG and LF were determined by quantitative rocket immunoelectrophoresis with application of research test systems. ALB, TP concentration – by biochemical methods (with bromcresol green and biuret blue). Level of cytokines (IL-8, IL-6, TNF-α, IFN-γ, VEGF) was determined by EIA method using appropriate commercial test systems. Results. Serum levels of studied regulatory and transport proteins and cytokines did not have statistically significant difference between groups of healthy fertile women and patients with primary and secondary infertility of male genesis. As a result of multifollicular ovarian stimulation and introduction of final oocytes maturation trigger in IVF programs among patients with male factor infertility significant reduction was identified of ALB, IL-8 concentration and advancement in VEGF on the date of transvaginal follicular puncture as compared to levels of the given parameters prior to program entry. Such changes were characteristic for both the subgroup of women with pregnancy occurrence in IVF program and the subgroup with failed treatment outcome. Conclusion. Absence of significant differences of studied parameters levels in blood serum between healthy fertile non-pregnant women and infertile patients with primary and secondary infertility of male genesis allowed developing a range of reference values of certain regulatory and transport proteins (α2-MG, α1-АТ, PAG, LF, ALB, TP) and cytokines (TNF-α, IL-6 and IL-8, IFN-γ, VEGF) for women under the age of 35 years with retained ovarian reserve. Dynamic reduction of ALB and IL-8 concentration, as well аs increase in VEGF level in IVF program among patients with male factor infertility without impact on outcome of the given programs can be assessed as normal organism reaction in response to multifollicular ovarian stimulation by follicle stimulation hormone agents and introduction of human chorionic gonadotropin as trigger of final oocytes maturation.
