Journal Title: Bioscience Reports
ISSN: 0144-8463 (Print); 1573-4935 (Online)
Publisher: Portland Press, Biochemical Society
Society/Institution: Biochemical Society
LCC Subject Category: Science: Biology (General): Life | Science: Microbiology
Country of publisher: United Kingdom
Language of fulltext: English
Full-text formats available: PDF, HTML
Department of Bioengineering, College of Materials and Chemistry Chemical Engineering, Chengdu University of Technology, Chengdu, 610059, China.
Guo‑ku Hu ( Department of Bioengineering, College of Materials and Chemistry Chemical Engineering, Chengdu University of Technology, Chengdu, 610059, China. )
Abstract | Full Text
Apoptosis mediates the precise and programmed natural death of neurons and is a physiologically important process in neurogenesis during maturation of the central nervous system. However, premature apoptosis and/or an aberration in apoptosis regulation are implicated in the pathogenesis of neurodegeneration. Thus, it is important to identify neuronal pathways/factors controlling apoptosis. Pink1 [phosphatase and tensin homologue (PTEN)-induced kinase 1] is a ubiquitously expressed gene and has been reported to have a physiological role in mitochondrial maintenance, suppressing mitochondrial oxidative stress, fission and autophagy. However, how Pink1 is involved in neuronal survival against oxidative stress remains not well understood. In the present paper, we demonstrate that thapsigargin, a specific irreversible inhibitor of endoplasmic reticulum (ER) calcium-ATPase, could lead to dramatic oxidative stress and neuronal apoptosis by ectopic calcium entry. Importantly, the neuronal toxicity of thapsigargin inhibits antioxidant gene Pink1 expression. Although Pink1 knockdown enhances the neuronal apoptosis by thapsigargin, its overexpression restores it. Our findings have established the neuronal protective role of Pink1 against oxidative stress and afford rationale for developing new strategy to the therapy of neurodegenerative diseases.