Compatibility study of Trichoderma harzianum Rifai and rice fungicides, and effects on three fungal plant pathogens

Abstract

This research took place at the Provincial Plant Sanitation Laboratory, in Camaguey, Cuba, between September 2013 and September 2015. The in vitro compatibility and antagonistic capacity of Trichoderma harzianum Rifai (strain A-34) on rice pathogens (Bipolaris oryzae Breda de Haan, Sarocladium oryzae (Sawada) w., Gams and D. Hawksworth and Magnaporthe grisea (Hebert Barr), was determined against pesticides used on rice. Assessment using traditional methods of microbiological isolation of mycelial growth, sporulation and conidial germination of the antagonist, to determine if the action mechanisms (antibiosis, competence, parasitism) against fungal pathogens, was made between 24 and 216 hours of application. A bifactorial design in dual culture was used for statistical analysis, along with scales for determination of microbial antagonistic capacity. Active ingredients tebuconazol + procloraz, trifloxistrobin+ ciproconazole, and epoxiconazole + kresoxim-methyl, affected mycelial growth of the antagonist. Moreover, the antagonist against active ingredients carbendazim, copper oxychloride, azoxystrobin and tebuconazo + triadimenol showed mycelial growth, sporulation and pathogen interaction, affecting their growth by means of coiling, penetration, granulation, and cell lysis, between 96 and 216 hours.

Keywords

• Trichoderma harzianum
• Bipolaris oryzae
• Sar o cladium oryzae
• Magnaporthe grisea
• Oryza sativa
• compatibility
• chemical fungicides
• bio pesticides
• rice