Communications Biology (Jul 2021)

A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia coli cells

  • Shengchen Wang,
  • Faying Zhang,
  • Meng Mei,
  • Ting Wang,
  • Yueli Yun,
  • Shihui Yang,
  • Guimin Zhang,
  • Li Yi

DOI
https://doi.org/10.1038/s42003-021-02374-w
Journal volume & issue
Vol. 4, no. 1
pp. 1 – 9

Abstract

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Wang et al. developed a fluorescence-assisted single-cell methodology (split protease-E. coli ClpXP (SPEC)) to characterise protein-protein interactions for both eukaryotic and prokaryotic species in E. coli cells. Their method can quantify these interactions with high sensitivity, easy manipulation, and broad temperature adaptability