Frequency and mechanisms of LINE-1 retrotransposon insertions at CRISPR/Cas9 sites

Jianli Tao; Qi Wang; Carlos Mendez-Dorantes; Kathleen H. Burns; Roberto Chiarle

doi:10.1038/s41467-022-31322-3

Nature Communications (Jun 2022)

Frequency and mechanisms of LINE-1 retrotransposon insertions at CRISPR/Cas9 sites

Jianli Tao,
Qi Wang,
Carlos Mendez-Dorantes,
Kathleen H. Burns,
Roberto Chiarle

Affiliations

Jianli Tao: Department of Pathology, Boston Children’s Hospital and Harvard Medical School
Qi Wang: Department of Pathology, Boston Children’s Hospital and Harvard Medical School
Carlos Mendez-Dorantes: Department of Oncologic Pathology, Dana-Farber Cancer Institute
Kathleen H. Burns: Department of Oncologic Pathology, Dana-Farber Cancer Institute
Roberto Chiarle: Department of Pathology, Boston Children’s Hospital and Harvard Medical School

DOI: https://doi.org/10.1038/s41467-022-31322-3
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 17

Abstract

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The identification of events occurring at target sites is critical to determine the safety of CRISPRbased DNA editing tools. Here, the authors show that LINE-1 retrotranspositions can occur frequently at canonical CRISPR/Cas9 editing sites, but are rare with prime editors and base editors.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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