Effect of resveratrol the expression of some genes involved epigenetic in breast cancer cell lines (MCF-7, MDA-MB-453)

Abstract

Background: Breast cancer is one of the most common cancers among women.  Incorrect pattern of gene expression involved in epigenetic  Including APOBEC3B, DNMT-1 and TET_1 can develop breast cancer.   resveratrol is a natural flavonoids that antioxidant and anti-cancer properties have been reported in other studies. To investigate the mechanism of effect of resveratrol, this study examined  effect of  resveratrol on the expression of genes referred to the two classes of  breast cancer cell lines Materials and methods: cell lines, including MCF-7 and MDA-MB-453 in separate boxes including the control group and treated groups with two doses of two doses of 25 and 100 micro molar for 24 hours of resveratrol for were cultured. RNA was extracted from the cells and then converted to cDNA. realtime PCR were used for APOBEC3B and DNMT and TET_1expression. Results: The results showed that resveratrol decrease in gene expression APOBEC3B and  DNMT-1 And increased expression of  TET_1 in both of cell lines.  . Discussion: due to satisfactory effects of resveratrol has on breast cancer cells, probably these effects  through  epigenetic mechanisms applied.  However, the final decision needs to be further investigation.

Keywords

• Resveratrol
• Gene expression
• Epigenetics
• Cell lines
• Breast cancer
• MCF-7
• MDA-MB-453