LncRNA 4930581F22Rik promotes myogenic differentiation by regulating the ERK/MAPK signaling pathway
Wei-Cai Chen,
Wan-Xin Chen,
Ye-Ya Tan,
Ying-Jun Xu,
Yi Luo,
Shi-Yu Qian,
Wan-Yi Xu,
Meng-Chun Huang,
Yan-Hua Guo,
Zhi-Gang Zhou,
Qi Zhang,
Jian-Xi Lu,
Shu-Juan Xie
Affiliations
Wei-Cai Chen
Biotherapy Center, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China
Wan-Xin Chen
Biotherapy Center, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China
Ye-Ya Tan
Guangzhou Women and Children's Medical Center, Guangdong Provincial Clinical Research Center for Child Health, Guangzhou, 510623, China
Ying-Jun Xu
Liver Disease Laboratory, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China
Yi Luo
Biotherapy Center, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China
Shi-Yu Qian
Department of Public Health and Preventive Medicine, School of Medicine, Jinan University, Guangzhou, 510632, China
Wan-Yi Xu
Biotherapy Center, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China
Meng-Chun Huang
Biotherapy Center, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China
Yan-Hua Guo
Guangzhou Quality Supervision and Testing Institute, Guangzhou, 511447, China
Zhi-Gang Zhou
Department of Orthopedics, First Affiliated Hospital, Jinan University, Guangzhou, 510630, China
Qi Zhang
Biotherapy Center, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China; Vaccine Research Institute of Sun Yat-sen University, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China
Jian-Xi Lu
Biotherapy Center, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China; Corresponding author. Biotherapy Center, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Shu-Juan Xie
Biotherapy Center, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China; Vaccine Research Institute of Sun Yat-sen University, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China; Corresponding author. Vaccine Research Institute of Sun Yat-sen University, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
The skeletal muscle is the largest organ in mammals and is the primary motor function organ of the body. Our previous research has shown that long non-coding RNAs (lncRNAs) are significant in the epigenetic control of skeletal muscle development. Here, we observed progressive upregulation of lncRNA 4930581F22Rik expression during skeletal muscle differentiation. Knockdown of lncRNA 4930581F22Rik hindered skeletal muscle differentiation and resulted in the inhibition of the myogenic markers MyHC and MEF2C. Furthermore, we found that lncRNA 4930581F22Rik regulates myogenesis via the ERK/MAPK signaling pathway, and this effect could be attenuated by the ERK-specific inhibitor PD0325901. Additionally, in vivo mice injury model results revealed that lncRNA 4930581F22Rik is involved in skeletal muscle regeneration. These results establish a theoretical basis for understanding the contribution of lncRNAs in skeletal muscle development and regeneration.
