Calcium Carbonate Nanoparticles—Toxicity and Effect of In Ovo Inoculation on Chicken Embryo Development, Broiler Performance and Bone Status
Arkadiusz Matuszewski,
Monika Łukasiewicz,
Jan Niemiec,
Maciej Kamaszewski,
Sławomir Jaworski,
Małgorzata Domino,
Tomasz Jasiński,
André Chwalibog,
Ewa Sawosz
Affiliations
Arkadiusz Matuszewski
Department of Animal Breeding, Institute of Animal Sciences, Warsaw University of Life Sciences (WULS–SGGW), 02-787 Warsaw, Poland
Monika Łukasiewicz
Department of Animal Breeding, Institute of Animal Sciences, Warsaw University of Life Sciences (WULS–SGGW), 02-787 Warsaw, Poland
Jan Niemiec
Department of Animal Breeding, Institute of Animal Sciences, Warsaw University of Life Sciences (WULS–SGGW), 02-787 Warsaw, Poland
Maciej Kamaszewski
Department of Ichthyology and Biotechnology in Aquaculture, Institute of Animal Sciences, Warsaw University of Life Sciences (WULS–SGGW), 02-787 Warsaw, Poland
Sławomir Jaworski
Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences (WULS-SGGW), 02-787 Warsaw, Poland
Małgorzata Domino
Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences (WULS–SGGW), 02-787 Warsaw, Poland
Tomasz Jasiński
Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences (WULS–SGGW), 02-787 Warsaw, Poland
André Chwalibog
Department of Veterinary and Animal Sciences, University of Copenhagen, Groennegaardsvej 3, 1870 Frederiksberg, Denmark
Ewa Sawosz
Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences (WULS-SGGW), 02-787 Warsaw, Poland
The use of intensive selection procedure in modern broiler chicken lines has led to the development of several skeletal disorders in broiler chickens. Therefore, current research is focused on methods to improve the bone quality in birds. In ovo technology, using nanoparticles with a high specificity to bones, is a potential approach. The present study aimed to evaluate the effect of in ovo inoculation (IOI) of calcium carbonate nanoparticles (CCN) on chicken embryo development, health status, bone characteristics, and on broiler production results and bone quality. After assessing in vitro cell viability, the IOI procedure was performed with an injection of 500 μg/mL CCN. The control group was not inoculated with CCN. Hatchability, weight, and selected bone and serum parameters were measured in embryos. Part of hatchlings were reared under standard conditions until 42 days, and production results, meat quality, and bone quality of broilers were determined. CCN did not show cytotoxicity to cells and chicken embryo and positively influenced bone parameters of the embryos and of broilers later (calcification) without negatively affecting the production results. Thus, the IOI of CCN could modify the molecular responses at the stage of embryogenesis, resulting in better mineralization, and could provide a sustained effect, thereby improving bone quality in adult birds.
