Mosaic Ends Tagmentation (METa) Assembly for Highly Efficient Construction of Functional Metagenomic Libraries

Terence S. Crofts; Alexander G. McFarland; Erica M. Hartmann

doi:10.1128/mSystems.00524-21

mSystems (Jun 2021)

Mosaic Ends Tagmentation (METa) Assembly for Highly Efficient Construction of Functional Metagenomic Libraries

Terence S. Crofts,
Alexander G. McFarland,
Erica M. Hartmann

Affiliations

Terence S. Crofts: ORCiD; Department of Molecular Biosciences, Northwestern University, Evanston, Illinois, USA
Alexander G. McFarland: ORCiD; Department of Civil and Environmental Engineering, Northwestern University, Evanston, Illinois, USA
Erica M. Hartmann: ORCiD; Department of Civil and Environmental Engineering, Northwestern University, Evanston, Illinois, USA

DOI: https://doi.org/10.1128/mSystems.00524-21
Journal volume & issue: Vol. 6, no. 3

Abstract

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Medically and industrially important genes can be recovered from microbial communities by high-throughput sequencing, but precise annotation is often limited to characterized genes and their relatives. Cloning a metagenome en masse

Published in mSystems

ISSN: 2379-5077 (Online)
Publisher: American Society for Microbiology
Country of publisher: United States
LCC subjects: Science: Microbiology
Website: https://journals.asm.org/journal/msystems

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