Química Nova (Jan 2008)

Desenvolvimento e validação de método por cromatografia líquida de alta eficiência para determinação simultânea das impurezas timina e timidina na matéria-prima estavudina Development and validation of a high performance liquid chromatographic method for simultaneous determination of the impurities thymine and thymidine in stavudine bulk drug

  • Gisele Rodrigues da Silva,
  • Felipe Antonacci Condessa,
  • Gérson Antônio Pianetti,
  • Elzíria de Aguiar Nunan,
  • Ligia Maria Moreira de Campos

DOI
https://doi.org/10.1590/s0100-40422008000700016
Journal volume & issue
Vol. 31, no. 7
pp. 1686 – 1690

Abstract

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A HPLC method was developed to quantify thymine and thymidine impurities in stavudine bulk drug. The separation was carried out in isocratic mode using methanol/water (20:80) as mobile phase, a C18 column and UV detection at 266 nm. The method provided selectivity based on peak purities and resolution among peaks. It was linear over the range of 0.5-5.0 µg/mL. The quantitation limits were 0.021 µg/mL for thymine and 0.134 µg/mL for thymidine. The average accuracies of three concentrations ranged from 97.06 to 102.61% and precision was close to 1%. The method showed robustness, remaining unaffected by deliberate variations in relevant parameters.

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