A human VE-cadherin-tdTomato and CD43-green fluorescent protein dual reporter cell line for study endothelial to hematopoietic transition

Ho Sun Jung; Gene Uenishi; Akhilesh Kumar; Mi Ae Park; Matt Raymond; Dustin Fink; Ethan McLeod; Igor Slukvin

doi:10.1016/j.scr.2016.09.004

Stem Cell Research (Sep 2016)

A human VE-cadherin-tdTomato and CD43-green fluorescent protein dual reporter cell line for study endothelial to hematopoietic transition

Ho Sun Jung,
Gene Uenishi,
Akhilesh Kumar,
Mi Ae Park,
Matt Raymond,
Dustin Fink,
Ethan McLeod,
Igor Slukvin

Affiliations

Ho Sun Jung: Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA
Gene Uenishi: Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA
Akhilesh Kumar: Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA
Mi Ae Park: Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA
Matt Raymond: Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA
Dustin Fink: Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA
Ethan McLeod: Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA
Igor Slukvin: Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA

DOI: https://doi.org/10.1016/j.scr.2016.09.004
Journal volume & issue: Vol. 17, no. 2
pp. 401 – 405

Abstract

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Human embryonic stem cell line WA01 was genetically modified using zinc-finger nucleases and the PiggyBac/transponson system to introduce a fluorescence reporter for VE-cadherin (VEC; tdTomato) and CD43 (eGFP). Phenotypic and functional assays for pluripotency revealed the modified hES cell reporter lines remained normal. When the cells were differentiated into hematoendothelial lineages, either by directed differentiation or direct reprogramming, flow cytometric and fluorescence microscopy showed that VEC+ endothelial cells express tdTomato and CD43+ hematopoietic progenitors express eGFP.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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