Fenofibrate induces HDL-associated PAF-AH but attenuates enzyme activity associated with apoB-containing lipoproteins

Vasilis Tsimihodimos; Anna Kakafika; Afroditi P. Tambaki; Eleni Bairaktari; M.John Chapman; Moses Elisaf; Alexandros D. Tselepis

Journal of Lipid Research (May 2003)

Fenofibrate induces HDL-associated PAF-AH but attenuates enzyme activity associated with apoB-containing lipoproteins

Vasilis Tsimihodimos,
Anna Kakafika,
Afroditi P. Tambaki,
Eleni Bairaktari,
M.John Chapman,
Moses Elisaf,
Alexandros D. Tselepis

Affiliations

Vasilis Tsimihodimos: Department of Internal Medicine, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Laboratory of Biological Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Medical School, and Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Institut National de la Santé et de la Recherché, Unité 551, Hôpital de la Pitié, 83 Bd de l’Hôpital, 75651 Paris Cedex 13, France
Anna Kakafika: Department of Internal Medicine, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Laboratory of Biological Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Medical School, and Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Institut National de la Santé et de la Recherché, Unité 551, Hôpital de la Pitié, 83 Bd de l’Hôpital, 75651 Paris Cedex 13, France
Afroditi P. Tambaki: Department of Internal Medicine, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Laboratory of Biological Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Medical School, and Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Institut National de la Santé et de la Recherché, Unité 551, Hôpital de la Pitié, 83 Bd de l’Hôpital, 75651 Paris Cedex 13, France
Eleni Bairaktari: Department of Internal Medicine, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Laboratory of Biological Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Medical School, and Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Institut National de la Santé et de la Recherché, Unité 551, Hôpital de la Pitié, 83 Bd de l’Hôpital, 75651 Paris Cedex 13, France
M.John Chapman: Department of Internal Medicine, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Laboratory of Biological Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Medical School, and Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Institut National de la Santé et de la Recherché, Unité 551, Hôpital de la Pitié, 83 Bd de l’Hôpital, 75651 Paris Cedex 13, France
Moses Elisaf: Department of Internal Medicine, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Laboratory of Biological Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Medical School, and Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Institut National de la Santé et de la Recherché, Unité 551, Hôpital de la Pitié, 83 Bd de l’Hôpital, 75651 Paris Cedex 13, France
Alexandros D. Tselepis: Department of Internal Medicine, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Laboratory of Biological Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Medical School, and Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece; Institut National de la Santé et de la Recherché, Unité 551, Hôpital de la Pitié, 83 Bd de l’Hôpital, 75651 Paris Cedex 13, France

Journal volume & issue: Vol. 44, no. 5
pp. 927 – 934

Abstract

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Human plasma platelet-activating factor acetylhydrolase (PAF-AH) is an enzyme associated mainly with the apolipoprotein B (apoB)-containing lipoproteins and primarily with LDL. A small proportion of enzymatic activity is also associated with HDL. Plasma paraoxonase 1 (PON1) is an esterase exclusively associated with HDL. The effect of fenofibrate on PAF-AH and PON1 activities in patients with dyslipidemias of Types IIA, IIB, and IV were studied. Fenofibrate reduced plasma PAF-AH activity in all patient groups. In Type IIA patients, this reduction was mainly due to a fall in enzyme activity associated with the dense LDL subspecies, whereas in Type IIB and Type IV patients, it was due to the decrease in PAF-AH activity associated with both the VLDL+IDL and dense LDL subspecies. Drug therapy in Type IIB and Type IV patients significantly increased the HDL-associated PAF-AH activity due to the increase in enzyme activity associated with the HDL-3c subfraction. Fenofibrate did not affect serum PON1 activities toward paraoxon and phenylacetate in either patient group.The fenofibrate-induced elevation of HDL-associated PAF-AH activity in dyslipidemic patients of Type IIB and Type IV, as well as the reduction in enzyme activity associated with atherogenic apoB-containing lipoproteins in all patient groups, may represent a new and important antiatherogenic effect of this potent lipid-modulating agent.

Published in Journal of Lipid Research

ISSN: 0022-2275 (Print); 1539-7262 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: https://www.journals.elsevier.com/journal-of-lipid-research

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