Calculating metalation in cells reveals CobW acquires CoII for vitamin B12 biosynthesis while related proteins prefer ZnII

Tessa R. Young; Maria Alessandra Martini; Andrew W. Foster; Arthur Glasfeld; Deenah Osman; Richard J. Morton; Evelyne Deery; Martin J. Warren; Nigel J. Robinson

doi:10.1038/s41467-021-21479-8

Nature Communications (Feb 2021)

Calculating metalation in cells reveals CobW acquires CoII for vitamin B12 biosynthesis while related proteins prefer ZnII

Tessa R. Young,
Maria Alessandra Martini,
Andrew W. Foster,
Arthur Glasfeld,
Deenah Osman,
Richard J. Morton,
Evelyne Deery,
Martin J. Warren,
Nigel J. Robinson

Affiliations

Tessa R. Young: Department of Biosciences, Durham University
Maria Alessandra Martini: Department of Biosciences, Durham University
Andrew W. Foster: Department of Biosciences, Durham University
Arthur Glasfeld: Department of Biosciences, Durham University
Deenah Osman: Department of Biosciences, Durham University
Richard J. Morton: Department of Mathematics, Physics, and Electrical Engineering, Northumbria University
Evelyne Deery: School of Biosciences, University of Kent
Martin J. Warren: School of Biosciences, University of Kent
Nigel J. Robinson: Department of Biosciences, Durham University

DOI: https://doi.org/10.1038/s41467-021-21479-8
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 15

Abstract

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The connection between metal binding to proteins and availabilities of different metals in cells has been unclear. Here, the authors report a metalation-calculator that takes into account competition between metals and their variable accessibility, and thereby elucidate in vivo metal occupancies of three different proteins, CobW, YeiR and YjiA.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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