Differential Inhibitory Effects of Curcumin Between HPV+ve and HPV–ve Oral Cancer Stem Cells

Nasreen Bano; Manisha Yadav; Bhudev C. Das

doi:10.3389/fonc.2018.00412

Frontiers in Oncology (Sep 2018)

Differential Inhibitory Effects of Curcumin Between HPV+ve and HPV–ve Oral Cancer Stem Cells

Nasreen Bano,
Manisha Yadav,
Bhudev C. Das

Affiliations

Nasreen Bano: Dr. B.R. Ambedkar Center for Biomedical Research, University of Delhi, New Delhi, India
Manisha Yadav: Dr. B.R. Ambedkar Center for Biomedical Research, University of Delhi, New Delhi, India
Bhudev C. Das: Stem Cell & Cancer Research Lab, Amity Institute of Molecular Medicine & Stem Cell Research, Amity University, Noida, India

DOI: https://doi.org/10.3389/fonc.2018.00412
Journal volume & issue: Vol. 8

Abstract

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Aim: To investigate the role of a herbal antioxidative compound curcumin on cell proliferation, orosphere formation and miRNA-21 expression in HPV16+ve/–ve oral cancer stem cells.Materials and Methods: Oral cancer stem cells were isolated from HPV+ve/HPV–ve oral cancer cell lines by FACS and stemness markers. MTT, spheroid assay and qRT-PCR were employed to examine the effects of curcumin.Results: Curcumin treatment in micromolar concentration (0–50 μM) demonstrated significant differential inhibition in CSC proliferation, orosphere formation and miRNA-21 expression in a dose dependent manner, the effect being highly pronounced in HPV positive CSCs.Conclusion: The strong and dose-dependent inhibitory effects of curcumin on cell proliferation, stemness and miRNA appear to be due to its chemosensitizing and anticancer effects on OSCC-CSCs.

Published in Frontiers in Oncology

ISSN: 2234-943X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: https://www.frontiersin.org/journals/oncology/

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