LncRNA HCP5 Promotes Cell Invasion and Migration by Sponging miR-29b-3p in Human Bladder Cancer

Abstract

Cheng Zhao, Yangle Li, Xiheng Hu, Ruizhe Wang, Wei He, Long Wang, Lin Qi, Shiyu Tong Department of Urology, Xiangya Hospital, Central South University, Changsha City, Hunan Province 410008, People’s Republic of ChinaCorrespondence: Shiyu TongDepartment of Urology, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha City, Hunan Province 410008, People’s Republic of ChinaEmail [email protected]: Bladder cancer (BC) is one of the most common malignant tumors in the urinary system. In this study, the roles of lncRNA HCP5 (human major histocompatibility complex p5) and miR-29b-3p in human BC were investigated. Their regulations involved in cell invasion and migration were also evaluated.Methods: Luciferase reporter assay was performed to detect the binding between miR-29b-3p and HCP5 or high-mobility group box 1 (HMGB1). Cell viability, migration, invasion and apoptosis were assessed by CCK-8, colony formation, transwell assay and flow cytometry, respectively. Expression levels of HMGB1/toll‐like receptor 4 (TLR4) proteins were measured by Western blot. Xenograft model was built, and tumor volumes and weights were calculated.Results: The results revealed dysregulation of HCP5 and miR-29b-3p in BC samples and cells. HCP5 negatively regulated the expression of miR-29b-3p and enhanced cell viability, migration and invasion. MiR-29b-3p mediated the effect of HCP5 on cell viability, proliferation, migration and invasion in RT4 cells. In addition, miR-29b-3p could regulate the expression of HMGB1 through interaction with HMGB1.Conclusion: The findings in this study supported that lncRNA HCP5 could promote cell invasion and migration by sponging miR-29b-3p in human BC.Keywords: lncRNA HCP5, invasions and migration, miR-29b-3p, human bladder cancer

Keywords

• lncrna hcp5
• invasions and migration
• mir-29b-3p
• human bladder cancer