PLoS ONE (Jan 2015)

Relationships between Micro-Vascular and Iodine-Staining Patterns in the Vicinity of the Tumor Front of Superficial Esophageal Squamous Carcinoma.

  • Shunsuke Ohta,
  • Kenro Kawada,
  • Jirawat Swangsri,
  • Naoto Fujiwara,
  • Katsumasa Saito,
  • Hisashi Fujiwara,
  • Tairo Ryotokuji,
  • Takuya Okada,
  • Yutaka Miyawaki,
  • Yutaka Tohkairin,
  • Yasuaki Nakajima,
  • Youichi Kumagai,
  • Kagami Nagai,
  • Takashi Ito,
  • Yoshinobu Eishi,
  • Tatsuyuki Kawano

DOI
https://doi.org/10.1371/journal.pone.0126533
Journal volume & issue
Vol. 10, no. 8
p. e0126533

Abstract

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The aim of the present study was to clarify differences between micro-vascular and iodine-staining patterns in the vicinity of the tumor fronts of superficial esophageal squamous cell carcinomas (ESCCs).Ten consecutive patients with ESCCs who were treated by endoscopic submucosal dissection (ESD) were enrolled. At the edge of the iodine-unstained area, we observed 183 sites in total using image-enhanced magnifying endoscopy. We classified the micro-vascular and iodine-staining patterns into three types: Type A, in which the line of vascular change matched the border of the iodine-unstained area; Type B, in which the border of the iodine-unstained area extended beyond the line of vascular change; Type C, in which the line of vascular change extended beyond the border of the iodine-unstained area. Then, by examining histopathological sections, we compared the diameter of intra-papillary capillary loops (IPCLs) in cancerous areas and normal squamous epithelium.We investigated 160 sites that the adequate quality of pictures were obtained. There was no case in which the line of vascular change completely matched the whole circumference of the border of an iodine-unstained area. Among the 160 sites, type A was recognized at 76 sites (47.5%), type B at 79 sites (49.4%), and type C at 5 sites (3.1%). Histological examination showed that the mean diameter of the IPCLs in normal squamous epithelium was 16.2±3.7 μm, whereas that of IPCLs in cancerous lesions was 21.0±4.4 μm.The development of iodine-unstained areas tends to precede any changes in the vascularity of the esophageal surface epithelium.