Structural Analysis of Wound Healing with Ehretia Laevis Roxb. Tincture Ointment versus Povidone-iodine Ointment in Wistar Rats: A Research Protocol

Abstract

Introduction: A wound is an opening in the skin’s epidermis that can lead to infection and sepsis. Individuals who experience injuries often report negative social, psychological, and physical effects as a result of their wounds and the treatment involved. Several factors can influence wound healing, including the site of the wound, medications, nutritional status, and bacterial infections. Ehretia laevis Roxb., a medicinal plant, is particularly beneficial for wound healing. It is commonly used by tribal communities for wound care, and they have reported positive outcomes. Additionally, this plant is also utilised to treat joint pain and minor fractures. Need of the study: Wound infections are frequently treated with potent antibiotics, which often remain inaccessible to rural populations. Furthermore, human resistance to these stronger drugs is increasing at an alarming rate, and patients are compelled to endure the adverse effects associated with such treatments. While povidone-iodine presents non allergic side effects, these cannot be overlooked. Notably, Ehretia laevis tincture ointment has not previously been formulated or employed in Ayurveda for wound healing. This study aims to develop a tincture-based ointment, providing a more standardised and clinically applicable form. Such an innovation may enhance both the practical usability and therapeutic efficacy of this natural remedy in wound care. Aim: To conduct a structural analysis of wound healing with Ehretia laevis Roxb. tincture ointment versus povidone-iodine ointment in Wistar rats, based on the percentage of wound reduction and histological parameters. Materials and Methods: An experimental preclinical study will be conducted in the central preclinical research facility of Jawaharlal Nehru Medical College, Datta Meghe Institute of Higher Education and Research, Sawangi (Meghe), Wardha, Maharashtra, India, from April 2025 to September 2025. The study will employ 12 albino Wistar rats of either sex, weighing between 150 and 200 grams. They will be divided into two groups, with six rats in each group. Each group will be kept in a separate enclosure with a 12-hour dark and light cycle in an animal house. The study will span a treatment period of 15 days, with follow-up assessments conducted on the 7th and 15th days. The outcomes will focus on wound contraction rates, histological evidence of tissue regeneration, and the overall healing timeline. A two-sample t-test will be employed to analyse quantitative data, while comparisons for qualitative data will be made using the Chi-square test. A p-value of less than 0.05 will be considered statistically significant.

Keywords

• amputation
• antibiotics
• infection
• wound contraction