DIFFERENTIAL PROFILE OF PRO-INFLAMMATSORY / ANTI-INFLAMMATORY CYTOKINES AND MALONDIALDEHYDE IN PATIENTS WITH ALCOtHOL-INDUCED OR OBESITY OR MIXED INJURY

Abstract

Introduction and Objectives: Dysregulation of pro-inflammatory/anti-inflammatory cytokines and oxidative stress markers has been reported in Non-Alcoholic Steatohepatitis (NASH) and alcoholic steatohepatitis (ASH); however, in the disease recently known, as Both Alcoholic and Non-Alcoholic SteatoHepatitis (BASH) that meets the criteria of ASH and NASH have not been described. The objective was to evaluate the influence of alcohol and obesity on a differential profile of cytokines and malondialdehyde (MDA) in patients with these etiologies. Material and methods: Cross-sectional, prospective, observational study. Patients from the “Dr. José E. González” from March 2019-March 2020, with a diagnosis of ASH (alcohol consumption ≥ 5 years, 30 g/day for men and 20 g/day for women), NASH (demonstrated by ultrasound, FibroScan or FibroMax) and BASH (ASH and NASH criteria). The serum cytokines interleukin-8 (IL-8), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), interleukin-10 (IL-10) and malondialdehyde (MDA) were determined. The protocol was approved by the ethics committee with registration MI19-000016.One-way analysis of variance was performed with Kruskal-Wallis and Dunn's post hoc. The results were expressed as median (interquartile range). The analysis was performed using Graph Pad Prism (v. 7.04, San Diego, CA, USA). A value of p<0.05 was considered significant. Results: The patients were: 34 BASH, 43 NASH and 35 ASH. The severity of the patients with respect to clinical and biochemical parameters in increasing order was NASH <BASH <ASH. It was observed in increasing order that the levels of the cytokines IL-8, IL-6, IL-10 were: NASH <BASH <ASH; TNF-α levels were: BASH <NASH <ASH; IL-1β levels show no significant difference between groups (Figure). The BASH group showed higher concentration levels of MDA [20.00 (9.00-30.50) pg/mL] with significant difference (p = 0.0434) compared to NASH [14.00 (3.00-19.00) pg/mL], but not with ASH (Figure). Serum levels of IL-6 (A; p <0.0001), TNF-α (B; p=0.0014), IL-8 (C; p<0.0001), IL-10 (D; p<0.0001) and IL-1β (E; Not significant) in the different study groups. Discussion: In ASH and NASH, common pathogenetic mechanisms mediated by pro-inflammatory.The authors declare that there is no conflict of interest.