A dual isotopic approach using radioactive phosphorus and the isotopic composition of oxygen associated to phosphorus to understand plant reaction to a change in P nutrition

Plant Methods. 2017;13(1):1-12 DOI 10.1186/s13007-017-0227-x

 

Journal Homepage

Journal Title: Plant Methods

ISSN: 1746-4811 (Online)

Publisher: BMC

LCC Subject Category: Agriculture: Plant culture | Science: Biology (General)

Country of publisher: United Kingdom

Language of fulltext: English

Full-text formats available: PDF, HTML

 

AUTHORS

Verena Pfahler (Department of Environmental Systems Science, ETH Zurich)
Federica Tamburini (Department of Environmental Systems Science, ETH Zurich)
Stefano M. Bernasconi (Department of Earth Sciences, ETH Zurich)
Emmanuel Frossard (Department of Environmental Systems Science, ETH Zurich)

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 11 weeks

 

Abstract | Full Text

Abstract Background Changing the phosphorus (P) nutrition leads to changes in plant metabolism. The aim of this study was to investigate how these changes are reflected in the distribution of 33P and the isotopic composition of oxygen associated to P (δ18OP) in different plant parts of soybean (Glycine max cv. Toliman). Two P pools were extracted sequentially with 0.3 M trichloroacetic acid (TCA P) and 10 M nitric acid (HNO3; residual P). Results The δ18OP of TCA P in the old leaves of the − P plants (23.8‰) significantly decreased compared to the + P plants (27.4‰). The 33P data point to an enhanced mobilisation of P from residual P in the old leaves of the − P plants compared to the + P plants. Conclusions Omitting P for 10 days lead to a translocation of P from source to sink organs in soybeans. This was accompanied by a significant lowering of the δ18OP of TCA P in the source organs due to the enzymatic hydrolysis of organic P. Combining 33P and δ18OP can provide useful insights in plant responses to P omission at an early stage.