Comparison of Antibiotic Resistance Profile between Salmonella Spp., Salmonella Enterica Ser. Typhimurium and Enteritidis and Escherichia Coli Isolated from Rectal Swabs of Chicken

Abstract

The aim of this experiment was comparing of antibiotic resistance profile between Salmonella spp. and Escherichia coli isolated from rectal swabs of chicken from conventional breeding. For the antibiotic susceptibility testing disk diffusion method was used. The both tested bacteria were exposed against thirteen antibiotics: ampicillin, piperacillin, cefotaxime, ceftriaxone, doripenem, meropenem, levofloxacin, ofloxacin, amikacin, gentamycin, tygecycline, tetracycline and chloramphenicol. For the identification of these strains, we used Chromogenic coliform agar, Triple sugar iron agar and biochemical test (ENTEROtest 24). We identified Salmonella spp. by used MicroSEQ® Salmonella spp. Detection Kit for identification of this strain in Step ONE Real Time PCR. In this study, we determined that Salmonella spp. was more resistant like Escherichia coli. The highest resistance had isolates of Salmonella spp. to levofloxacin (100%) and to ofloxacin (100%). Also to ampicillin was resistance in Salmonella spp. isolates about 83%. Only in case of piperacillin was resistance in Salmonella spp. isolates lower (50%) like in Escherichia coli isolates (66.6%). The both strains were 100 % sensitive to doripenem, meropenem, amikacin, gentamycin and tygecycline. Antibiotic resistance is a biological danger. Bacteria, which we study, are considered to reservoirs of resistant genes and they are facultative and obligate pathogens. If these pathogen bacteria cause diseases those these diseases are difficult to treat.

Keywords

• antibiotic resistance
• chicken
• escherichia coli
• rectal
• salmonella spp