Quantitative determination of hepatic and lipoprotein lipase activities from human postheparin plasma1

J Boberg; J Augustin; M L Baginsky; P Tejada; W V Brown

Journal of Lipid Research (Jul 1977)

Quantitative determination of hepatic and lipoprotein lipase activities from human postheparin plasma1

J Boberg,
J Augustin,
M L Baginsky,
P Tejada,
W V Brown

Affiliations

J Boberg: Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093; Department of Geriatrics, University of Uppsala, Box 641, S-751 27 Uppsala, Sweden
J Augustin: Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093
M L Baginsky: Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093
P Tejada: Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093
W V Brown: Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093

Journal volume & issue: Vol. 18, no. 4
pp. 544 – 547

Abstract

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A method was developed to separate and quantitatively determine two different triglyceride lipase activities in human postheparin plasma: hepatic triglyceride lipase (H-TGL) and lipoprotein lipase (LPL). Affinity chromatography on heparin-Sepharose columns was used for the separation. Rechromatography of purified H-TGL on heparin-Sepharose resulted in recoveries of 74 and 97% of these enzyme activities, respectively. The analytical errors for the determinations of the two activities were 11.4 and 9.6%, respectively.

triglyceride lipase activities

Published in Journal of Lipid Research

ISSN: 0022-2275 (Print); 1539-7262 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: https://www.journals.elsevier.com/journal-of-lipid-research

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Abstract

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