Transcriptomic Analysis Identified ARHGAP Family as a Novel Biomarker Associated With Tumor-Promoting Immune Infiltration and Nanomechanical Characteristics in Bladder Cancer

Chen Yang; Chen Yang; Chen Yang; Siqi Wu; Siqi Wu; Zezhong Mou; Zezhong Mou; Quan Zhou; Quan Zhou; Zheyu Zhang; Zheyu Zhang; Yiling Chen; Yiling Chen; Yuxi Ou; Yuxi Ou; Xinan Chen; Xinan Chen; Xiyu Dai; Xiyu Dai; Chenyang Xu; Chenyang Xu; Na Liu; Haowen Jiang; Haowen Jiang; Haowen Jiang

doi:10.3389/fcell.2021.657219

Frontiers in Cell and Developmental Biology (Jul 2021)

Transcriptomic Analysis Identified ARHGAP Family as a Novel Biomarker Associated With Tumor-Promoting Immune Infiltration and Nanomechanical Characteristics in Bladder Cancer

Chen Yang,
Chen Yang,
Chen Yang,
Siqi Wu,
Siqi Wu,
Zezhong Mou,
Zezhong Mou,
Quan Zhou,
Quan Zhou,
Zheyu Zhang,
Zheyu Zhang,
Yiling Chen,
Yiling Chen,
Yuxi Ou,
Yuxi Ou,
Xinan Chen,
Xinan Chen,
Xiyu Dai,
Xiyu Dai,
Chenyang Xu,
Chenyang Xu,
Na Liu,
Haowen Jiang,
Haowen Jiang,
Haowen Jiang

Affiliations

Chen Yang: Department of Urology, Huashan Hospital, Fudan University, Shanghai, China
Chen Yang: Fudan Institute of Urology, Huashan Hospital, Fudan University, Shanghai, China
Chen Yang: National Clinical Research Center for Aging and Medicine, Fudan University, Shanghai, China
Siqi Wu: Department of Urology, Huashan Hospital, Fudan University, Shanghai, China
Siqi Wu: Fudan Institute of Urology, Huashan Hospital, Fudan University, Shanghai, China
Zezhong Mou: Department of Urology, Huashan Hospital, Fudan University, Shanghai, China
Zezhong Mou: Fudan Institute of Urology, Huashan Hospital, Fudan University, Shanghai, China
Quan Zhou: Department of Urology, Huashan Hospital, Fudan University, Shanghai, China
Quan Zhou: Fudan Institute of Urology, Huashan Hospital, Fudan University, Shanghai, China
Zheyu Zhang: Department of Urology, Huashan Hospital, Fudan University, Shanghai, China
Zheyu Zhang: Fudan Institute of Urology, Huashan Hospital, Fudan University, Shanghai, China
Yiling Chen: Department of Urology, Huashan Hospital, Fudan University, Shanghai, China
Yiling Chen: Fudan Institute of Urology, Huashan Hospital, Fudan University, Shanghai, China
Yuxi Ou: Department of Urology, Huashan Hospital, Fudan University, Shanghai, China
Yuxi Ou: Fudan Institute of Urology, Huashan Hospital, Fudan University, Shanghai, China
Xinan Chen: Department of Urology, Huashan Hospital, Fudan University, Shanghai, China
Xinan Chen: Fudan Institute of Urology, Huashan Hospital, Fudan University, Shanghai, China
Xiyu Dai: Department of Urology, Huashan Hospital, Fudan University, Shanghai, China
Xiyu Dai: Fudan Institute of Urology, Huashan Hospital, Fudan University, Shanghai, China
Chenyang Xu: Department of Urology, Huashan Hospital, Fudan University, Shanghai, China
Chenyang Xu: Fudan Institute of Urology, Huashan Hospital, Fudan University, Shanghai, China
Na Liu: School of Mechatronics Engineering and Automation, Shanghai University, Shanghai, China
Haowen Jiang: Department of Urology, Huashan Hospital, Fudan University, Shanghai, China
Haowen Jiang: Fudan Institute of Urology, Huashan Hospital, Fudan University, Shanghai, China
Haowen Jiang: National Clinical Research Center for Aging and Medicine, Fudan University, Shanghai, China

DOI: https://doi.org/10.3389/fcell.2021.657219
Journal volume & issue: Vol. 9

Abstract

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Bladder cancer (BCa) is a common lethal urinary malignancy worldwide. The role of ARHGAP family genes in BCa and its association with immuno-microenvironment remain largely unknown. ARHGAP family expression and immune infiltration in BCa were analyzed by bioinformatics analysis. Then, we investigated cell proliferation, invasion, and migration in vivo and in vitro of the ARHGAP family. Furthermore, atomic force microscopy (AFM) was employed in measuring cellular mechanical properties of BCa cells. The results demonstrated that ARHGAP family genes correlate with a tumor-promoting microenvironment with a lower Th1/Th2 cell ratio, higher DC cell infiltration, higher Treg cell infiltration, and T-cell exhaustion phenotype. Silencing ARHGAP5, ARHGAP17, and ARHGAP24 suppressed BCa cell proliferation, migration, and metastasis. Knocking down of ARHGAPs in T24 cells caused a relatively higher Young’s modulus and lower adhesive force and cell height. Taken together, ARHGAP family genes promote BCa progressing through establishing a tumor-promoting microenvironment and promoting cancer progression.

Published in Frontiers in Cell and Developmental Biology

ISSN: 2296-634X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: https://www.frontiersin.org/journals/cell-and-developmental-biology

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