Modified CRISPR/Cas9 mediated generation of two MKK7 knockout human embryonic stem cell lines

C.H. Cole Sims; Matias I. Autio; Adriana Buskin; Christine Cheung; Rakesh Heer; Roger S.Y. Foo; Xin Wang

Stem Cell Research (Apr 2021)

Modified CRISPR/Cas9 mediated generation of two MKK7 knockout human embryonic stem cell lines

C.H. Cole Sims,
Matias I. Autio,
Adriana Buskin,
Christine Cheung,
Rakesh Heer,
Roger S.Y. Foo,
Xin Wang

Affiliations

C.H. Cole Sims: Translational and Clinical Research Institute, Newcastle University, UK
Matias I. Autio: Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore; Cardiovascular Disease Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
Adriana Buskin: Translational and Clinical Research Institute, Newcastle University, UK
Christine Cheung: Nanyang Technological University, Singapore
Rakesh Heer: Translational and Clinical Research Institute, Newcastle University, UK
Roger S.Y. Foo: Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore; Cardiovascular Disease Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
Xin Wang: Division of Cardiovascular Sciences, The University of Manchester, UK; Corresponding author.

Journal volume & issue: Vol. 52
p. 102238

Abstract

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Two cell lines were generated by CRISPR/Cas9 mediated knockout of MKK7 (MAP2K7) by removal of exon 1 or exons 4 through 7. These knockouts were confirmed at the transcript and protein levels. These hESCs are pluripotent and maintain tri-lineage differentiation capacity. These cell lines are a useful resource for studying MKK7 function in humans.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal