Characteristics of Novel Chicken Embryonic Stem Cells Established Using Chicken Leukemia Inhibitory Factor

Mikiharu Nakano; Kenjiro Arisawa; Satomi Yokoyama; Masaki Nishimoto; Yusuke Yamashita; Maya Sakashita; Ryo Ezaki; Haruo Matsuda; Shuichi Furusawa; Hiroyuki Horiuchi

doi:10.2141/jpsa.010102

The Journal of Poultry Science (Jan 2011)

Characteristics of Novel Chicken Embryonic Stem Cells Established Using Chicken Leukemia Inhibitory Factor

Mikiharu Nakano,
Kenjiro Arisawa,
Satomi Yokoyama,
Masaki Nishimoto,
Yusuke Yamashita,
Maya Sakashita,
Ryo Ezaki,
Haruo Matsuda,
Shuichi Furusawa,
Hiroyuki Horiuchi

Affiliations

Mikiharu Nakano: Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, Japan
Kenjiro Arisawa: Hiroshima Prefectural Institute of Industrial Science and Technology, Hiroshima Industrial Promotion Organization, Japan
Satomi Yokoyama: Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, Japan
Masaki Nishimoto: Hiroshima Prefectural Institute of Industrial Science and Technology, Hiroshima Industrial Promotion Organization, Japan
Yusuke Yamashita: Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, Japan
Maya Sakashita: Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, Japan
Ryo Ezaki: Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, Japan
Haruo Matsuda: Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, Japan
Shuichi Furusawa: Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, Japan
Hiroyuki Horiuchi: Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, Japan

DOI: https://doi.org/10.2141/jpsa.010102
Journal volume & issue: Vol. 48, no. 1
pp. 64 – 72

Abstract

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Chickens are an ideal animal model study tool for developmental biology, and a farm animal with excellent productivity. Researchers have therefore long sought to establish chicken embryonic stem cells (cESCs) to enable the creation of genetically modified chickens. Here, we derived novel cESCs from chicken blastodermal cells (CBCs) cultured with chicken leukemia inhibitory factor (chLIF). These cESCs have the capacity for long-term (100 days or more) successive subculture and express both chicken Nanog (chNanog) and chicken vasa homolog (Cvh) mRNAs and proteins. The cESCs showed a capacity for chimeric formation during a transplant experiment that used a fertilized egg. Transfer of the enhanced green fluorescent protein (EGFP) gene to cESCs enabled green fluorescence to be observed among primordial germ cells (PGCs). These results indicate that novel cESCs should have the capacity to differentiate into germ cells.

Published in The Journal of Poultry Science

ISSN: 1346-7395 (Print); 1349-0486 (Online)
Publisher: Japan Poultry Science Association
Country of publisher: Japan
LCC subjects: Agriculture: Animal culture
Website: https://jpn-psa.jp/en/jps-en/

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