Journal of Experimental Pharmacology (Mar 2023)
Pharmacological Evaluation of the Anesthetic and Analgesic Potential of Injection Harsha 22: A Novel Polyherbal Local Anesthetic Formulation Intended for Parenteral Administration in Wistar Albino Rats
Abstract
Shan Sasidharan,1,2 Asha Nair Kaveri,3 M S Sithara,2 Hareendran Nair J4 1Department of R&D, Pankajakasthuri Herbal Research Foundation, Thiruvananthapuram, Kerala, India; 2Small Animal Research Centre, Department of Toxicology and Pharmacology, CARe KERALA, Thrissur, Kerala, India; 3Department of Shalyatantra, Pankajakasthuri Ayurveda Medical College & P.G. Centre, Thiruvananthapuram, Kerala, India; 4Pankajakasthuri Herbal India Pvt. Ltd, Thiruvananthapuram, Kerala, IndiaCorrespondence: Shan Sasidharan, Pankajakasthuri Herbal Research Foundation, Thiruvananthapuram, Kerala, India, Email [email protected]: Local anaesthetics are medications that cause numbness that can be reversed by applying them topically. Local anaesthetics are clinically used to control pain during minor surgeries or to treat other acute and chronic pain. The present investigation intended to investigate the anesthetic as well as analgesic potential of Injection Harsha 22, a novel polyherbal formulation in Wistar albino rats.Methods: The anesthetic potential of Injection Harsha 22 was evaluated by a heat tail-flick latency (TFL) test, whereas the analgesic effect was elevated by electrical stimulation testing. Here, lignocaine (2%) was used as the standard anesthetic drug.Results: In TFL, Injection Harsha 22 showed anesthetic effects up to 90 minutes after application. Also, the duration of anesthesia in rats that were administered subcutaneously with Injection Harsha 22 was comparable to that of the rats treated with commercial lignocaine (2%). In an electrical stimulation test, single administration of Injection Harsha 22 to rats significantly prolonged analgesia compared with the normal control group. The median duration of analgesia in rats administered subcutaneously with Injection Harsha 22 and lignocaine solution was 40 minutes and 35 minutes, respectively. Furthermore, Injection Harsha 22 does not interfere with the hematopoietic system of the experiment animals.Conclusion: Thus, the present investigation established the in vivo anesthetic and analgesic potential of Injection Harsha 22 in experimental animals. Hence, it can be concluded that Injection Harsha 22 can become a prominent substitute for lignocaine as a local anaesthetic agent after establishing its efficacy through stringent clinical trials in humans.Keywords: anesthetic, analgesic, local anesthetic, polyherbal anesthetic formulation, Wistar rats
