Application and research progress of recombinase isothermal amplification technology in the analysis of animal and plant components

Abstract

Recently， the analysis of animal- and plant-derived components， mainly based on the adulteration and smuggling of meat products and genetically modified crops， has been a key focus in the field of food safety. Therefore， the requirement for analytical methods has been increasingly emphasized. Recombinase isothermal amplification is a novel and promising detection technique that currently includes recombinase polymerase amplification （RPA） and recombinase-aided amplification （RAA）. This technique has been widely used in animal- and plant-derived component analysis and other detection and analysis fields owing to its speed， convenience， and high sensitivity and specificity. This paper summarizes relevant research conducted locally and abroad in recent years and concludes with an analysis of various detection methods. It also generalizes the characteristics and applications of RPA/RAA technology in four aspects： primer-probe design， external factors， reaction devices， and detection methods， and lays out the prospects for RPA/RAA development trends. Finally， this study also provides a reference for improving the analysis and detection methods of animal- and plant-derived components and promoting RPA/RAA technology.

Keywords

• recombinase amplification technology
• animal and plant sources
• rapid detection