Cryogenic contrast-enhanced microCT enables nondestructive 3D quantitative histopathology of soft biological tissues

Arne Maes; Camille Pestiaux; Alice Marino; Tim Balcaen; Lisa Leyssens; Sarah Vangrunderbeeck; Grzegorz Pyka; Wim M. De Borggraeve; Luc Bertrand; Christophe Beauloye; Sandrine Horman; Martine Wevers; Greet Kerckhofs

doi:10.1038/s41467-022-34048-4

Nature Communications (Oct 2022)

Cryogenic contrast-enhanced microCT enables nondestructive 3D quantitative histopathology of soft biological tissues

Arne Maes,
Camille Pestiaux,
Alice Marino,
Tim Balcaen,
Lisa Leyssens,
Sarah Vangrunderbeeck,
Grzegorz Pyka,
Wim M. De Borggraeve,
Luc Bertrand,
Christophe Beauloye,
Sandrine Horman,
Martine Wevers,
Greet Kerckhofs

Affiliations

Arne Maes: Department of Materials Engineering, KU Leuven
Camille Pestiaux: Biomechanics lab, Institute of Mechanics, Materials and Civil Engineering, UCLouvain
Alice Marino: Pole of Cardiovascular Research, Institute of Experimental and Clinical Research, UCLouvain
Tim Balcaen: Biomechanics lab, Institute of Mechanics, Materials and Civil Engineering, UCLouvain
Lisa Leyssens: Biomechanics lab, Institute of Mechanics, Materials and Civil Engineering, UCLouvain
Sarah Vangrunderbeeck: Biomechanics lab, Institute of Mechanics, Materials and Civil Engineering, UCLouvain
Grzegorz Pyka: Biomechanics lab, Institute of Mechanics, Materials and Civil Engineering, UCLouvain
Wim M. De Borggraeve: Molecular Design and Synthesis, Department of Chemistry, KU Leuven
Luc Bertrand: Pole of Cardiovascular Research, Institute of Experimental and Clinical Research, UCLouvain
Christophe Beauloye: Division of Cardiology, University Hospital Saint-Luc
Sandrine Horman: Pole of Cardiovascular Research, Institute of Experimental and Clinical Research, UCLouvain
Martine Wevers: Department of Materials Engineering, KU Leuven
Greet Kerckhofs: Department of Materials Engineering, KU Leuven

DOI: https://doi.org/10.1038/s41467-022-34048-4
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 14

Abstract

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The authors present cryogenic contrast-enhanced MicroCT (cryo-CECT), which by freezing stained samples at optimal freezing rates improves the visualization of the tissue microstructure. They demonstrate quantitative 3D analysis of individual tissue constituents, such as muscle and collagen fibers.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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